Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PINK1 antibody [EPR20730] (ab216144)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20730] to PINK1
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-PINK1 antibody [EPR20730]
    See all PINK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20730] to PINK1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cells (+/- treatment with 10uM carbonyl cyanide 3-chlorophenylhydrazone (CCCP, ab141229) for 24 hours) whole cell lysate; human PINK1 recombinant protein (aa156-507) . ICC/IF: HeLa cells treated with 10uM carbonyl cyanide 3-chlorophenylhydrazone (CCCP, ab141229) for 24 hours. IP: HeLA cells (treated with 10uM carbonyl cyanide 3-chlorophenylhydrazone (CCCP, ab141229) for 24 hours) whole cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PINK1 antibody [EPR20730] (ab216144)
    Western blot - Anti-PINK1 antibody [EPR20730] (ab216144)
    Anti-PINK1 antibody [EPR20730] (ab216144) at 1/1000 dilution + Human PINK1 recombinant protein (aa156-507), 10 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

    Developed using the ECL technique.

    Predicted band size: 63 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Dilution/blocking buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR20730] (ab216144)
    Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR20730] (ab216144)

    Immunofluorescent analysis of 4 % paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma)(+/- treatment with 10μM carbonyl cyanide 3-chlorophenylhydrazone (CCCP, ab141229) for 24 hours) cells labeling PINK1 with ab216144 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells treated with 10μM carbonyl cyanide 3-chlorophenylhydrazone (CCCP, ab141229) for 24 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    The negative controls are as follows:
    -ve control: PBS, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-PINK1 antibody [EPR20730] (ab216144)
    Western blot - Anti-PINK1 antibody [EPR20730] (ab216144)
    All lanes : Anti-PINK1 antibody [EPR20730] (ab216144) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa cells (treated with 10uM carbonyl cyanide 3-chlorophenylhydrazone (CCCP, ab141229) for 24 hours) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 63 kDa
    Observed band size: 62 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and dilution buffer: 5% NFDM/TBST

    PINK1 can be induced by CCCP treatment (PMID: 24184327).

  • Immunoprecipitation - Anti-PINK1 antibody [EPR20730] (ab216144)
    Immunoprecipitation - Anti-PINK1 antibody [EPR20730] (ab216144)

    PINK1 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) (treated with 10uM carbonyl cyanide 3-chlorophenylhydrazone (CCCP. ab141229) for 24 hours) whole cell lysate with ab216144 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216144 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution.

    Lane 1: HeLa (CCCP-treated, ab141229) lysate 10 μg (Input).
    Lane 2: ab216144 IP in HeLa (CCCP-treated, ab141229) lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216144 in HeLa (CCCP-treated, ab141229) whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-PINK1 antibody [EPR20730] (ab216144)
    Anti-PINK1 antibody [EPR20730] (ab216144)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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