Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free (ab248011)
![Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free (ab248011)](https://www.abcam.com/ps/products/248/ab248011/Images/ab248011-408178-anti-niemann-pick-c1-like-1-npc1l1-antibody-epr5717-bsa-and-azide-free-western-blot.jpg "Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free (ab248011)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5717] to Niemann Pick C1 Like 1/NPC1L1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free
See all Niemann Pick C1 Like 1/NPC1L1 primary antibodies -
Description
Rabbit monoclonal [EPR5717] to Niemann Pick C1 Like 1/NPC1L1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248011 is the carrier-free version of ab124801 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248011 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Niemann Pick C1 Like 1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5717 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free (ab248011)Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801) at 1/1000 dilution (purified) + HeLa whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDaThis data was developed using ab124801, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free (ab248011)](https://www.abcam.com/ps/products/248/ab248011/Images/ab248011-406256-anti-niemann-pick-c1-like-1-npc1l1-antibody-epr5717-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free (ab248011)Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801) at 1/10000 dilution (purified) + Human fetal liver tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDaThis data was developed using ab124801, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free (ab248011)](https://www.abcam.com/ps/products/248/ab248011/Images/ab248011-403014-anti-niemann-pick-c1-like-1-npc1l1-antibody-epr5717-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free (ab248011)All lanes : Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal liver lysates
Lane 2 : HeLa lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 149 kDaThis data was developed using ab124801, the same antibody clone in a different buffer formulation.
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![Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free (ab248011)](https://www.abcam.com/ps/products/248/ab248011/Images/ab248011-4-benefits-of-recombinant-antibodies.png)
Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free (ab248011)