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Anti-CLIC4 antibody [EPR14253] (ab183043)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14253] to CLIC4
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CLIC4 antibody [EPR14253]
    See all CLIC4 primary antibodies

  • Description

    Rabbit monoclonal [EPR14253] to CLIC4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, 293, Raji and HepG2 lysates; Human melanoma tissue; HeLa, HepG2 and Raji cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Western blot - Anti-CLIC4 antibody [EPR14253] (ab183043)
    All lanes : Anti-CLIC4 antibody [EPR14253] (ab183043) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : 293 lysate
    Lane 3 : Raji lysate
    Lane 4 : HepG2 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 29 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (ab183043)

    Immunofluorescence analysis of HeLa cells (-20°C Acetone-fixed) labeling CLIC4 with ab183043 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC4 antibody [EPR14253] (ab183043)

    Immunohistochemical analysis of paraffin embedded Human melanoma tissue labeling CLIC4 with ab183043 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Immunoprecipitation - Anti-CLIC4 antibody [EPR14253] (ab183043)

    Western blot analysis of immunoprecipitation pellet from Human Raji cell lysate immunoprecipitated using ab183043 at 1/50 dilution.
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary antibody used at 1/1500 dilution.

  • Flow Cytometry - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Flow Cytometry - Anti-CLIC4 antibody [EPR14253] (ab183043)

    Flow cytometric analysis of Raji cells (paraformaldehyde-fixed, 2%) labeling CLIC4 with ab183043 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (ab183043)

    Immunofluorescence analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling CLIC4 with ab183043 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Anti-CLIC4 antibody [EPR14253] (ab183043)
    Anti-CLIC4 antibody [EPR14253] (ab183043)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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