Anti-PINK1 antibody (ab23707)
Key features and details
- Rabbit polyclonal to PINK1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PINK1 antibody
See all PINK1 primary antibodies -
Description
Rabbit polyclonal to PINK1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic peptide:
LVRALLQREA SKRPSARVAA N
, corresponding to amino acids 484-504 of Human PINK1. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINK1 antibody (ab23707)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling PINK1 with ab23707 at 1/200 dilution. Slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen (DAB). Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-PINK1 antibody (ab23707)Anti-PINK1 antibody (ab23707) at 4 µg/ml + Murine liver 100,000 x g pellet at 30 µg
Observed band size: 66 kDa why is the actual band size different from the predicted?
Additional bands at: 33 kDa (possible cleavage fragment)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINK1 antibody (ab23707) This image is courtesy of an Abreview submitted by Mr. Allan Wang.Immunohistochemistry (Formaldehyde-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissues labelling PINK1 with ab23707 at 1/100 dilution (28 minutes at 37°C). Heat mediated antigen retrieval was performed using a buffer at pH 8.5. An HRP-conjugated Goat Anti-rabbit polyclonal was used as the secondary antibody.
