Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PINK1 antibody [EPR20730] - BSA and Azide free (ab232374)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20730] to PINK1 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-PINK1 antibody [EPR20730] - BSA and Azide free
    See all PINK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20730] to PINK1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells treated with 10uM carbonyl cyanide 3-chlorophenylhydrazone (CCCP) for 24 hours.

  • General notes

    Ab232374 is the carrier-free version of ab216144. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232374 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-PINK1 antibody [EPR20730] - BSA and Azide free (ab232374)
    Immunoprecipitation - Anti-PINK1 antibody [EPR20730] - BSA and Azide free (ab232374)

    PINK1 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma)(treated with 10uM carbonyl cyanide 3-chlorophenylhydrazone (CCCP) for 24 hours) whole cell lysate with ab216144 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216144 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.


    Lane 1: HeLa (CCCP-treated) lysate 10 μg (Input).
    Lane 2: ab216144 IP in HeLa (CCCP-treated) lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216144 in HeLa (CCCP-treated) whole cell lysate.


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216144).

  • Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR20730] - BSA and Azide free (ab232374)
    Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR20730] - BSA and Azide free (ab232374)

    Immunofluorescent analysis of 4 % paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma)(+/- treatment with 10μM carbonyl cyanide 3-chlorophenylhydrazone (CCCP) for 24 hours) cells labeling PINK1 with ab216144 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells treated with 10μM carbonyl cyanide 3-chlorophenylhydrazone (CCCP) for 24 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).


    The negative controls are as follows:
    -ve control: PBS, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216144).

  • Anti-PINK1 antibody [EPR20730] - BSA and Azide free (ab232374)
    Anti-PINK1 antibody [EPR20730] - BSA and Azide free (ab232374)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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