Anti-PINK1 antibody [EPR20730] - BSA and Azide free (ab232374)
![Anti-PINK1 antibody [EPR20730] - BSA and Azide free (ab232374)](https://www.abcam.com/ps/products/232/ab232374/Images/ab232374-321358-anti-pink1-antibody-epr20730-immunoprecipitation.jpg "Anti-PINK1 antibody [EPR20730] - BSA and Azide free (ab232374)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20730] to PINK1 - BSA and Azide free
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Overview
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Product name
Anti-PINK1 antibody [EPR20730] - BSA and Azide free
See all PINK1 primary antibodies -
Description
Rabbit monoclonal [EPR20730] to PINK1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HeLa cells treated with 10uM carbonyl cyanide 3-chlorophenylhydrazone (CCCP) for 24 hours.
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General notes
Ab232374 is the carrier-free version of ab216144. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab232374 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20730 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-PINK1 antibody [EPR20730] - BSA and Azide free (ab232374)
PINK1 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma)(treated with 10uM carbonyl cyanide 3-chlorophenylhydrazone (CCCP) for 24 hours) whole cell lysate with ab216144 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216144 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: HeLa (CCCP-treated) lysate 10 μg (Input).
Lane 2: ab216144 IP in HeLa (CCCP-treated) lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216144 in HeLa (CCCP-treated) whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216144).
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![Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR20730] - BSA and Azide free (ab232374)](https://www.abcam.com/ps/products/232/ab232374/Images/ab232374-308927-anti-pink1-antibody-epr20730-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR20730] - BSA and Azide free (ab232374)Immunofluorescent analysis of 4 % paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma)(+/- treatment with 10μM carbonyl cyanide 3-chlorophenylhydrazone (CCCP) for 24 hours) cells labeling PINK1 with ab216144 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells treated with 10μM carbonyl cyanide 3-chlorophenylhydrazone (CCCP) for 24 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
The negative controls are as follows:
-ve control: PBS, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216144).
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![Anti-PINK1 antibody [EPR20730] - BSA and Azide free (ab232374)](https://www.abcam.com/ps/products/232/ab232374/Images/ab232374-3-benefits-of-recombinant-antibodies.png)
Anti-PINK1 antibody [EPR20730] - BSA and Azide free (ab232374)
