Anti-PCDH15 antibody [EPR19600] - BSA and Azide free (ab251375)
![Anti-PCDH15 antibody [EPR19600] - BSA and Azide free (ab251375)](https://www.abcam.com/ps/products/251/ab251375/Images/ab251375-2-anti-pcdh15-antibody-epr19600-bsa-and-azide-free-immunocytochemistry.jpg "Anti-PCDH15 antibody [EPR19600] - BSA and Azide free (ab251375)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19600] to PCDH15 - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Mouse
Overview
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Product name
Anti-PCDH15 antibody [EPR19600] - BSA and Azide free
See all PCDH15 primary antibodies -
Description
Rabbit monoclonal [EPR19600] to PCDH15 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251375 is the carrier-free version of ab202560. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251375 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19600 -
Isotype
IgG
Images
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Immunocytochemistry - Anti-PCDH15 antibody [EPR19600] - BSA and Azide free (ab251375)This data was developed using ab202560, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with a GFP-tagged mouse PCDH15 expression construct or GFP vector only, labeling PCDH15 with ab202560 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red). Confocal image showing positive staining on HEK-293 cells transfected with a GFP-tagged mouse PCDH15 expression construct. This mouse PCDH15 plasmid was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150115 (Alexa Fluor ® 647 Goat anti-Mouse secondary) at 1/1000 dilution (white). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 594) (ab150080) at 1/1000 dilution.
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![Western blot - Anti-PCDH15 antibody [EPR19600] - BSA and Azide free (ab251375)](https://www.abcam.com/ps/products/251/ab251375/Images/ab251375-399196-anti-pcdh15-antibody-epr19600-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-PCDH15 antibody [EPR19600] - BSA and Azide free (ab251375)All lanes : Anti-PCDH15 antibody [EPR19600] (ab202560) at 1/1000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with an empty expression vector
Lane 2 : 293T whole cell lysate transfected with a DDDK-tagged mouse PCDH15 construct
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 215 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis data was developed using ab202560, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 16481439).
The mouse PCDH15 plasmid was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.
WB detection of endogenous PCDH15 is not shown due to unavailability of appropriate testing material.
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![Anti-PCDH15 antibody [EPR19600] - BSA and Azide free (ab251375)](https://www.abcam.com/ps/products/251/ab251375/Images/ab251375-3-benefits-of-recombinant-antibodies.png)
Anti-PCDH15 antibody [EPR19600] - BSA and Azide free (ab251375)