Anti-Klotho antibody [EPR6856] (ab181373)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6856] to Klotho
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Klotho antibody [EPR6856]
See all Klotho primary antibodies -
Description
Rabbit monoclonal [EPR6856] to Klotho -
Host species
Rabbit -
Specificity
When we tested the antibody in human samples, the only form of the protein that we are able to detect is the secreted one (about 65kDa). When we tested the antibody in mouse samples (wt vs KO) we could only detect the membrane bound form of the protein (about 130kDa). This difference could be due to the nature of the samples (different species). However, we do not have a clear explanation about this. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented here. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseHuman -
Immunogen
Synthetic peptide within Human Klotho aa 400-500. The exact sequence is proprietary.
Database link: Q9UEF7 -
Positive control
- Human testis, fetal kidney and fetal thymus lysates; Human clear cell carcinoma kidney tissue, Human kidney tissue.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6856 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Klotho antibody [EPR6856] (ab181373) at 5 µg/ml
Lane 1 : Wild Type (WT) Mouse Kidney Tissue Lysate
Lane 2 : Knock Out (KO) Mouse Kidney Tissue Lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 62, 116 kDaThe blots were produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane were blocked for an hour before being incubated with ab181373 (rabbit anti-Klotho antibody; diluted 5 ug/ml) for 24 hours at 4°C. Antibody binding was detected using infrared labelled goat anti-rabbit (green) antibody at 1:10,000 dilutions for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Klotho with unpurified ab181373 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Klotho antibody [EPR6856] (ab181373) at 1/1000 dilution (purified)
Lane 1 : Human fetal kidney
Lane 2 : Human testis
Lane 3 : Human fetal thymus
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 62, 116 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunohistochemical staining of paraffin embedded human kidney with purified ab181373 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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All lanes : Anti-Klotho antibody [EPR6856] (ab181373) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal kidney lysates
Lane 2 : Human testis lysates
Lane 3 : Human fetal thymus lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 62, 116 kDa
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Immunohistochemical analysis of paraffin-embedded human kidney clear cell carcinoma tissue labeling Klotho with unpurified ab181373 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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