Anti-COASY antibody (ab226400)
Key features and details
- Rabbit polyclonal to COASY
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-COASY antibody
See all COASY primary antibodies -
Description
Rabbit polyclonal to COASY -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human COASY aa 225-275. The exact sequence is proprietary.
Database link: Q13057 -
Positive control
- IP: Jurkat whole cell lysate.
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General notes
ab226400 has not performed satisfactorily when used for WB of COASY in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of COASY.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226400 was affinity purified using an epitope specific to COASY immobilized on solid support -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-COASY antibody (ab226400)
COASY was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226400 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226400 at 1 µg/ml.
Lane 1: ab226400 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
