Anti-PGC1 beta antibody [EPR12370] (ab176328)
![Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.com/ps/products/176/ab176328/Images/ab176328-257234-anti-pgc1-beta-antibody-epr12370-western-blot.jpg "Anti-PGC1 beta antibody [EPR12370] (ab176328)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12370] to PGC1 beta
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-PGC1 beta antibody [EPR12370]
See all PGC1 beta primary antibodies -
Description
Rabbit monoclonal [EPR12370] to PGC1 beta -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PGC1 beta aa 950 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q86YN6 -
Positive control
- WB: HeLa, MCF-7 and SH-SY5Y cell lysates and human fetal brain, human fetal heart, rat spleen and mouse spleen tissue lysates. IP: HeLa whole cell lysate (ab150035).
-
General notes
This product may be covered by one or more of the following U.S. Patents.
6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.5% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12370 -
Isotype
IgG -
Research areas
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Images
-
Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)All lanes : Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/10000 dilution (purified)
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF-7 whole cell lysate
Lane 3 : SH-SY5Y whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 113 kDa
Observed band size: 113 kDa
Blocking and dilution buffer: 5% NFDM/TBST. -
![Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.com/ps/products/176/ab176328/Images/ab176328-257235-anti-pgc1-beta-antibody-epr12370-western-blot.jpg)
Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)All lanes : Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/10000 dilution (purified)
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 113 kDa
Observed band size: 113 kDa
Blocking and dilution buffer: 5% NFDM/TBST. -
![Immunoprecipitation - Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.com/ps/products/176/ab176328/Images/ab176328-257199-anti-pgc1-beta-antibody-epr12370-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PGC1 beta antibody [EPR12370] (ab176328)ab176328 (purified) at 1/20 immunoprecipitating PGC1 beta in HeLa whole cell lysate.
Lane 1 (input): HeLa whole cell lysate (10µg)
Lane 2 (+): ab176328 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab176328 in HeLa whole cell lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.com/ps/products/176/ab176328/Images/ab176328-204140-anti-pgc1-beta-antibody-epr12370-western-blot.jpg)
Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)All lanes : Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Human fetal brain tissue lysate
Lane 4 : Human fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 113 kDa
-
![Immunoprecipitation - Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.com/ps/products/176/ab176328/Images/ab176328-204143-anti-pgc1-beta-antibody-epr12370-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PGC1 beta antibody [EPR12370] (ab176328)Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling PGC1 beta using unpurified ab176328 at a dilution of 1/10.
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![Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.com/ps/products/176/ab176328/Images/ab176328-8-benefits-of-recombinant-antibodies.png)
Anti-PGC1 beta antibody [EPR12370] (ab176328)
