Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HGF antibody [EPR12230] (ab178395)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12230] to HGF
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-HGF antibody [EPR12230]
    See all HGF primary antibodies

  • Description

    Rabbit monoclonal [EPR12230] to HGF

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human HGF aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: P14210

  • Positive control

    • Human placenta and U-87 MG cell lysates and Human HGF (aa 32-728) recombinant protein. IP: Human placenta lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-HGF antibody [EPR12230] (ab178395)
    Western blot - Anti-HGF antibody [EPR12230] (ab178395)
    Anti-HGF antibody [EPR12230] (ab178395) at 1/1000 dilution (Purified) + U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

  • Western blot - Anti-HGF antibody [EPR12230] (ab178395)
    Western blot - Anti-HGF antibody [EPR12230] (ab178395)
    Anti-HGF antibody [EPR12230] (ab178395) at 1/1000 dilution (Purified) + Human HGF (aa 32-728) recombinant protein

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 83 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-HGF antibody [EPR12230] (ab178395)
    Western blot - Anti-HGF antibody [EPR12230] (ab178395)
    Anti-HGF antibody [EPR12230] (ab178395) at 1/200 dilution (Purified) + Human placenta lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

  • Immunoprecipitation - Anti-HGF antibody [EPR12230] (ab178395)
    Immunoprecipitation - Anti-HGF antibody [EPR12230] (ab178395)

    ab178395 (purified) at 1:30 dilution (2µg) immunoprecipitating HGF in Human placenta lysate.
    Lane 1 (input): Human placenta lysate 10µg
    Lane 2 (+): ab178395 & Human placenta lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab178395 in Human placenta lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Anti-HGF antibody [EPR12230] (ab178395)
    Anti-HGF antibody [EPR12230] (ab178395)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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