This data was developed using ab181298, the same antibody clone in a different buffer formulation.HEK-293 cells were co-transfected with a plasmid encoding the full-length mouse leptin receptor, pRL-CMV and pGL4.47[luc2P/SIE/Hygro] Vectors. Dual-Luciferase® Reporter Assay was used to test the leptin signaling.ab181298 (0, 50, 100 µg/ml) was incubated with recombinant mouse leptin in a total of 500ul culture medium at the final concentration of 100, 50, 25, and 12.5 ng/ml for 2h at 37°C before adding to transfected cells.Leptin without incubating with ab181298 as the positive control (green) shows the maximal signal of the leptin and leptin receptor interaction, whereas the base line signal (without adding leptin and ab181298 to the system) serves as the negative control (purple).The data demonstrates that the interaction of leptin and leptin receptor can be blocked by ab181298 resulting in decreased florescence signals.

This data was developed using ab181298, the same antibody clone in a different buffer formulation.HEK-293 cells were co-transfected with a plasmid encoding full length human leptin receptor, pRL-CMV and pGL4.47[luc2P/SIE/Hygro] Vectors. Dual-Luciferase® Reporter Assay was used to test the leptin signaling. ab181298 (0, 50, 100 µg/ml) was incubated with recombinant mouse leptin in a total of 500ul culture medium at the final concentration of 100, 50, 25, and 12.5 ng/ml for 2h at 37? before adding to transfected cells. Leptin without incubating with ab181298 as the positive control (green) shows the maximal signal of the leptin and leptin receptor interaction, whereas the base line signal (without adding leptin and ab181298 to the system) serves as the negative control (purple). The data demonstrates that the interaction of leptin and leptin receptor can be blocked by ab181298 resulting in decreased florescence signals. The data was provided by our collaborator Dr. Bing Sun, Chinese Academy of Sciences.