Human Resistin ELISA Kit (RETN) (ab108896)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.25 ng/ml - 16 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Resistin ELISA Kit (RETN)
See all Resistin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.3% Inter-assay Sample n Mean SD CV% Overall 10.2% -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.2 ng/ml -
Range
0.25 ng/ml - 16 ng/ml -
Recovery
= 97 %
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Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Resistin (RETN) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Resistin concentrations in Human plasma, serum, urine, saliva and cell culture supernatants.
A Resistin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Resistin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Resistin captured in plate.
Get results in 90 minutes with Human Resistin ELISA Kit (ab183364) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Resistin Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Resistin Microplate (12 x 8 well strips) 1 unit Resistin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. -
Tissue specificity
Expressed only in fatty tissues. -
Sequence similarities
Belongs to the resistin/FIZZ family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Adipose tissue specific secretory factor
- Adipose tissue-specific secretory factor
- ADSF
see all -
Database links
- Entrez Gene: 56729 Human
- Omim: 605565 Human
- SwissProt: Q9HD89 Human
- Unigene: 283091 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab108896.
