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Human Resistin ELISA Kit (ab183364)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 24 pg/ml
  • Range: 78.1 pg/ml - 5000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Resistin ELISA Kit
    See all Resistin kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 9 3%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 6%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    24 pg/ml

  • Range

    78.1 pg/ml - 5000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 94 88% - 100%
    Cell culture media 106 106% - 107%
    Hep Plasma 95 84% - 110%
    EDTA Plasma 96 88% - 104%
    Cit plasma 78 68% - 90%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Resistin ELISA kit (ab183364) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Resistin protein in human serum, plasma and cell culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Resistin with 24 pg/mL sensitivity.

    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips

    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

  • Notes

    Resistin, otherwise known as Found in Inflammatory Zone 3 (FIZZ3), and Adipocyte Secreted Factor (ADSF), is a 114 amino-acid cysteine-rich protein. It can be found as a high molecular weight hexamer or as a more active, lower molecular weight complex. Resistin is the best known member of the family of Resistin-like Molecules (RELMs), which include RELM-alpha/FIZZ1 and RELM-beta/FIZZE 2, both described only in rodents. Furthermore, resistin has been categorized as part of the group of adipokines hormones, which are known to be involved in the development of obesity, insulin resistance, diabetes, inflammation, autoimmune disease and metabolic syndrome.

    In Humans, Resistin is a pro-inflammatory molecule primarily secreted by macrophages and mononuclear bone marrow cells in response to inflammation, hyperglycemia, and growth hormones. IL-1, IL-6, TNF-alpha as well as lipopolysaccharide (LPS) are strong inducers of resistin as observed in animal models of hepatic inflammation and necrosis. High levels of resistin are also observed in patients with pancreatitis and with systemic lupus erythematosus.

  • Platform

    Microplate

Properties

Images

  • Other - Human Resistin ELISA Kit (ab183364)
    Other - Human Resistin ELISA Kit (ab183364)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of Resistin standard curve.
    Example of Resistin standard curve.

    Background-subtracted data values (mean +/- SD) are graphed

  • Levels of Resistin in Normal Serum.
    Levels of Resistin in Normal Serum.

    The levels of native Resistin in serum samples were tested from ten individual healthy donors. Levels were interpolated from the standard curve in Sample Diluent NS. The mean level of Resistin was 2.6 ng/mL with a range of 1.6 to 5.3 ng/mL and a standard deviation of 1 ng/mL.

  • Example of Resistin standard curve in cell culture media.
    Example of Resistin standard curve in cell culture media.

    Background-subtracted data values (mean +/- SD) are graphed.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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