Anti-Gephyrin antibody (ab185993)
Key features and details
- Rabbit polyclonal to Gephyrin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Gephyrin antibody
See all Gephyrin primary antibodies -
Description
Rabbit polyclonal to Gephyrin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
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Immunogen
Synthetic peptide within Human Gephyrin aa 250-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_001019389.1.
Database link: Q9NQX3 -
Positive control
- HeLa, 293T and Jurkat cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab185993 was affinity purified using an epitope specific to Gephyrin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Gephyrin antibody (ab185993)
Detection of Gephyrin in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab185993 at 6 µg/mg lysate for IP (Lane 1). For WB detection, ab185993 was used at 1µg/ml. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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Western blot - Anti-Gephyrin antibody (ab185993)All lanes : Anti-Gephyrin antibody (ab185993) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 80 kDa
Exposure time: 3 minutes
