Anti-Gephyrin antibody (ab228674)
Key features and details
- Rabbit polyclonal to Gephyrin
- Suitable for: WB, IP, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Gephyrin antibody
See all Gephyrin primary antibodies -
Description
Rabbit polyclonal to Gephyrin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatIHC-Fr RatIHC-P MouseIP HumanWB MouseHuman
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Immunogen
Recombinant fragment within Human Gephyrin (internal sequence). The exact sequence is proprietary.
Database link: Q9NQX3 -
Positive control
- IHC-Fr: E13.5 rat brain tissue. WB: U-87 MG, SK-N-SH, IMR32, NIH/3T3, JC and SK-N-AS whole cell extracts; mouse brain tissue extract; rat brain tissue extract. IHC-P: Mouse brain tissue. IP: A431 whole cell extract. ICC/IF: DIV10 rat E18 primary hippocampal neurons.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Gephyrin antibody (ab228674)All lanes : Anti-Gephyrin antibody (ab228674) at 1/3000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extracts
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extracts
Lane 3 : IMR32 (human neuroblast cell line) whole cell extracts
Lane 4 : SK-N-AS (human neuroblastoma cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 80 kDa7.5% SDS-PAGE
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Immunohistochemistry (Frozen sections) - Anti-Gephyrin antibody (ab228674)Frozen sectioned E13.5 rat brain tissue stained for Gephyrin with ab228674 at 1/250 dilution (green) in immunohistochemical analysis.
Beta Tubulin 3/ TUJ1, a mature neuron marker, is detected with a beta Tubulin 3/ TUJ1 antibody (red). Nuclear counterstain: Fluoroshield with DAPI (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody (ab228674)Paraffin-embedded mouse brain tissue stained for Gephyrin with ab228674 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-Gephyrin antibody (ab228674)All lanes : Anti-Gephyrin antibody (ab228674) at 1/5000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 80 kDa7.5% SDS-PAGE
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Western blot - Anti-Gephyrin antibody (ab228674)All lanes : Anti-Gephyrin antibody (ab228674) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : JC (mouse mammary gland adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 80 kDa7.5% SDS-PAGE
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Immunoprecipitation - Anti-Gephyrin antibody (ab228674)Gephyrin was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 µg ab228674. Western blot was performed from the immunoprecipitate using ab228674.
Lane 2: Control IgG IP in A431 whole cell extract.
Lane 1: ab228674 IP in A431 whole cell extract.
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Immunocytochemistry/ Immunofluorescence - Anti-Gephyrin antibody (ab228674)DIV10 rat E18 primary hippocampal neurons stained for Gephyrin (green) using ab228674 (1:500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500 (red). Fluoroshield with DAPI (blue).
