Anti-PD1 antibody [J116] (ab140950)
Key features and details
- Mouse monoclonal [J116] to PD1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PD1 antibody [J116]
See all PD1 primary antibodies -
Description
Mouse monoclonal [J116] to PD1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Human PD1 protein
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Positive control
- Human tonsil tissue; Human PD1 transfected cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.88% Sodium chloride, 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
J116 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [J116] (ab140950)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labelling PD1 with ab140950 at 20 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [J116] (ab140950) This image is courtesy of an anonymous Abreview.
ab140950 staining PD1 in human nasopharynx tissue sections by Immunohistochemistry (Formalin/PFA-fixed frozen paraffin-embedded sections). Tissue samples were fixed with paraformaldehyde and blocked with 4% serum for 30 minutes at 25°C and antigen retrieval was by heat mediation in citrate buffer (pH 6.0). The sample was incubated with primary antibody (20ug/ml) in blocking buffer at 4°C for 16 hours. An Alexa Fluor® 488-conjugated Goat anti-mouse polyclonal (1/100) was used as the secondary antibody.