Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PD1 antibody [CAL15] (ab237727)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAL15] to PD1
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-PD1 antibody [CAL15]
    See all PD1 primary antibodies

  • Description

    Rabbit monoclonal [CAL15] to PD1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MOLT-4 (treated with PMA (10ng/ml 24h) and Ionomycin (500ng/ml 24h) whole cell lysate; human tonsil and thymus lysates. IHC-P: Human tonsil and spleen tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [CAL15] (ab237727)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [CAL15] (ab237727)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PD1 with ab237727 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the human tonsil is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab237727 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [CAL15] (ab237727)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [CAL15] (ab237727)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling PD1 with ab237727 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the human spleen is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab237727 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-PD1 antibody [CAL15] (ab237727)
    Western blot - Anti-PD1 antibody [CAL15] (ab237727)
    All lanes : Anti-PD1 antibody [CAL15] (ab237727) at 1/1000 dilution

    Lane 1 : Untreated MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
    Lane 2 : MOLT-4 (treated with PMA (10ng/ml 24h) and Ionomycin (500ng/ml 24h) whole cell lysate
    Lane 3 : Human tonsil lysate
    Lane 4 : Human thymus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 32 kDa
    Observed band size: 50-55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.
    The expression profile observed is consistent with what has been described in the literature (PMID: 26318293).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [CAL15] (ab237727)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [CAL15] (ab237727)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for PD1 using ab237727 at 0.25 µg/ml dilution in immunohistochemical analysis.

  • Anti-PD1 antibody [CAL15] (ab237727)
    Anti-PD1 antibody [CAL15] (ab237727)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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