Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸ 1 ml 2 680 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [CAL15] to PD1
Suitable for: IHC-P, WB
Reacts with: Human

Product name

Anti-PD1 antibody [CAL15]
See all PD1 primary antibodies
Description

Rabbit monoclonal [CAL15] to PD1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MOLT-4 (treated with PMA (10ng/ml 24h) and Ionomycin (500ng/ml 24h) whole cell lysate; human tonsil and thymus lysates. IHC-P: Human tonsil and spleen tissue.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.50
Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein A purified
Purification notes

Purity >99%
Clonality

Monoclonal
Clone number

CAL15
Isotype

IgG
Research areas
- Cancer
- Cell Death
- Apoptosis
- Other

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [CAL15] (ab237727)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PD1 with ab237727 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the human tonsil is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab237727 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [CAL15] (ab237727)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling PD1 with ab237727 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the human spleen is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab237727 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Western blot - Anti-PD1 antibody [CAL15] (ab237727)

All lanes : Anti-PD1 antibody [CAL15] (ab237727) at 1/1000 dilution

Lane 1 : Untreated MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 2 : MOLT-4 (treated with PMA (10ng/ml 24h) and Ionomycin (500ng/ml 24h) whole cell lysate
Lane 3 : Human tonsil lysate
Lane 4 : Human thymus lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 32 kDa
Observed band size: 50-55 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.
The expression profile observed is consistent with what has been described in the literature (PMID: 26318293).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [CAL15] (ab237727)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for PD1 using ab237727 at 0.25 µg/ml dilution in immunohistochemical analysis.
Anti-PD1 antibody [CAL15] (ab237727)

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