Antibodies, Proteins, Kits and Reagents for Life Science

345 091 ₸ Product size

100 µl 345 091 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PKM antibody [EPR10138(B)] (ab150377)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10138(B)] to PKM
Suitable for: ICC/IF, WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-PKM antibody [EPR10138(B)]
See all PKM primary antibodies
Description

Rabbit monoclonal [EPR10138(B)] to PKM
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human PKM aa 50-150. The exact sequence is proprietary.
Database link: P14618
Positive control
- WB: Human skeletal muscle, HeLa, Jurkat and A549 cell lysates. Mouse brain, heart, kidney, and spleen tissue lysates. Rat brain, heart, and spleen tissue lysates; ICC/IF: HeLa cells; Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10138(B)
Isotype

IgG
Research areas
- Cancer
- Tumor biomarkers
- Enzymes
- Other

Western blot - Anti-PKM antibody [EPR10138(B)] (ab150377)

All lanes : Anti-PKM antibody [EPR10138(B)] (ab150377) at 1/1000 dilution

Lane 1 : Human skeletal muscle cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A549 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit antibody at 1/2000 dilution

Predicted band size: 58 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody [EPR10138(B)] (ab150377)

Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling PKM with purified ab150377 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

Control: PBS only
Western blot - Anti-PKM antibody [EPR10138(B)] (ab150377)

All lanes : Anti-PKM antibody [EPR10138(B)] (ab150377) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 58 kDa

Exposure time: 180 seconds

Blocking buffer: 5% NFDM/TBST
Flow Cytometry - Anti-PKM antibody [EPR10138(B)] (ab150377)

Flow cytometric analysis of permeabilized HeLa cells labeling PKM with ab150377 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Anti-PKM antibody [EPR10138(B)] (ab150377)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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