Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric) (ab107923)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 1 µM
Overview
-
Product name
Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric)
See all Glucose 6 phosphate kits -
Detection method
Fluorescent -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 1 µM -
Range
1 µM - 500 µM -
Assay time
0h 20m -
Product overview
Abcam's Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric) (ab107923) is a simple, sensitive and rapid means of quantifying glucose-6-Phosphate (G6P) in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product that converts a nearly colorless probe to an intensely fluorescent product (Ex/Em 535/587 nm). This assay can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 µM in the original sample assuming a dilution of 5X during processing. This Glucose-6-Phosphate Assay Kit - High Sensitivity is more sensitive than our colorimetric assay, Glucose-6-Phosphate Assay Kit (ab83426).
Visit our FAQs page for tips and troubleshooting. -
Notes
Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells which then enters either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NAD(P)H. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Storage buffer
Preservative: None
Constituents: DMSO -
Components Identifier 100 tests Glucose 6 phosphate assay buffer WM 1 x 25ml Glucose 6 phosphate Enzyme Mix (lyophilized) Green 1 vial Glucose 6 phosphate Standard (10 µmol; lyophilized) Yellow 1 vial Glucose 6 phosphate Substrate Mix (lyophilized) Red 1 vial Probe (in DMSO) Blue 1 x 0.4ml -
Research areas
-
Relevance
Glucose 6 phosphate (G6P) is a key sugar intermediate for glucose to get into cells, and then enter either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. -
Alternative names
- G6P
Images
-
Functional Studies - Glucose 6 phosphate Assay Kit(ab107923)
Glucose 6 phosphate measured in mouse tissue lysates showing quantity (pmol) per mg protein of tested sample
-
Functional Studies - Glucose 6 phosphate Assay Kit (ab107923)Glucose 6 phosphate measured in biological fluids showing quantity (pmol) per microliter of tested sample
-
Functional Studies - Glucose 6 phosphate Assay Kit (ab107923)Glucose 6 phosphate standard curves (0 – 500 and 0 – 50 pmol range) generated using ab107923.
