Glucose-6-Phosphate Assay Kit (Colorimetric) (ab83426)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 10 µM
Overview
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Product name
Glucose-6-Phosphate Assay Kit (Colorimetric)
See all Glucose 6 phosphate kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
10 µM -
Range
1 µM - 30 µM -
Assay time
0h 40m -
Product overview
Glucose-6-Phosphate Assay Kit (Colorimetric) (ab83426) is a simple, sensitive and rapid test to quantify glucose-6-phospate (G6P) in a variety of samples. In the assay, glucose-6-phosphate is oxidized into an intermediate product which in turn converts the probe into an intensely colored product with an absorbance at OD=450 nm. This product can detect G6P in the range of 1-30 nmoles with a detection sensitivity ~10 µM G6P.
For higher sensitivity, we recommend our fluorometric assay Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric) (ab107923).
Visit our FAQs page for tips and troubleshooting. -
Notes
Glucose-6-phosphate (G6P) is a key sugar intermediate for glucose to get into cells, and then enter either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is transformed by G6PDH to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests G6P Assay Buffer WM 1 x 25ml G6P Enzyme Mix (Lyophilised) Green 1 vial G6P Standard (10 µmol) (Lyophilised) Yellow 1 vial G6P Substrate Mix (Lyophilised) Red 1 vial -
Research areas
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Relevance
Glucose 6 phosphate (G6P) is a key sugar intermediate for glucose to get into cells, and then enter either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. -
Alternative names
- G6P
Images
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Functional Studies - Glucose 6 Phosphate Assay Kit (ab83426) Image from Taylor KM et al., PLoS One. 2013;8(2):e56181. Fig 6(A).; doi: 10.1371/journal.pone.0056181. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
G6P (A) levels are elevated in GAA−/− mice compared to wild type (C57/Bl6) and reduced by rhGAA treatment.
GAA−/− mice were dosed with rhGAA. G6P was quantified in heart and triceps homogenates from wild type (C57Bl/6) and GAA−/− mice using ab83426. Values are means ± SEM. Data was analyzed by one-way ANOVA followed by Newman-Keuls comparing groups. ***P
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Functional Studies - Glucose 6 Phosphate Assay Kit (ab83426)Colorimetric standard curve: mean of duplicates (+/- SD) with background reads subtracted.
