Anti-GLP1 antibody (ab22625)
Key features and details
- Rabbit polyclonal to GLP1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GLP1 antibody
See all GLP1 primary antibodies -
Description
Rabbit polyclonal to GLP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide corresponding to Human GLP1 aa 1-19 conjugated to Bovine Serum Albumin (BSA) (Bis-diazotised tolidine (Bdt)). The mid to C terminal region of GLP 1 [1-19] enabling immunoreactivity with N terminal truncated and C terminally extended forms of GLP 1 (e.g. GLP 1[7 to 36]amide).
Sequence:HDEFERHAEGTFTSDVSSY
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody (ab22625)ab22625 (1/2000) staining GLP1 in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining in the pancreatic islets.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
