Anti-GLP1 antibody [1B7B4] (ab36598)
Key features and details
- Mouse monoclonal [1B7B4] to GLP1
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-GLP1 antibody [1B7B4]
See all GLP1 primary antibodies -
Description
Mouse monoclonal [1B7B4] to GLP1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Ni-NTA purified truncated recombinant GLP expressed in E. Coli strain BL21 (DE3).
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Positive control
- IHC-P (FFPE): Human Pancreas (Normal)
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR286968-1 are from Tissue Culture Supernatant
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1B7B4 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [1B7B4] (ab36598)
IHC image of GLP1 staining in Human Pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36598, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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All lanes : Anti-GLP1 antibody [1B7B4] (ab36598)
Lane 1 : Molecular weight marker
Lane 2 : Truncated GLP recombinant protein
Predicted band size: 21 kDa
Observed band size: ~20 kDa why is the actual band size different from the predicted?