Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GLP1 antibody [EPR4042-1] (ab108443)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4042-1] to GLP1
  • Suitable for: ELISA, ICC/IF, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-GLP1 antibody [EPR4042-1]
    See all GLP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4042-1] to GLP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Glicentin polypeptide, Human pancreas tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)

    Unpurified ab108443, at 1/100 dilution, staining Glicentin in Human pancreas tissue by immunohistochemistry

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)
    ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)

    1 ug/mL of Glucagon-like Peptide (GLP-1) was coated into 96-wells. Various concentrations of anti-Glucagon-Like Peptide 1 (GLP-1, 7-36,amide) rabbit monoclonal antibody (unpurified ab108443) was added. HRP conjugated goat anti-rabbit IgG antibody was used to develop the color.

  • Immunocytochemistry/ Immunofluorescence - Anti-GLP1 antibody [EPR4042-1] (ab108443)
    Immunocytochemistry/ Immunofluorescence - Anti-GLP1 antibody [EPR4042-1] (ab108443)
    Immunofluorescence staining of BxPC-3 cells with purified ab108443 at a working dilution of 1/800, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108443 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)
    Immunohistochemical staining of paraffin embedded human pancreas with purified ab108443 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Western blot - Anti-GLP1 antibody [EPR4042-1] (ab108443)
    Western blot - Anti-GLP1 antibody [EPR4042-1] (ab108443)
    Anti-GLP1 antibody [EPR4042-1] (ab108443) at 1/2000 dilution (purified) + human fetal pancreas lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 11 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)
    Immunohistochemical staining of paraffin embedded human liver with purified ab108443 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)
    ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)

    Antigen dose-response curve using purified ab108443. Antibody concentration of 1 µg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)
    ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)

    Antibody dose-response curve using purified ab108443. Antigen concentration of 1 µg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • Western blot - Anti-GLP1 antibody [EPR4042-1] (ab108443)
    Western blot - Anti-GLP1 antibody [EPR4042-1] (ab108443)
    Anti-GLP1 antibody [EPR4042-1] (ab108443) at 1/200 dilution (unpurified) + Glicentin polypeptide at 0.005 µg

    Predicted band size: 21 kDa

  • Anti-GLP1 antibody [EPR4042-1] (ab108443)
    Anti-GLP1 antibody [EPR4042-1] (ab108443)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-GLP1 antibody [EPR4042-1] (ab108443)

© 2021 Astana Biomed Group LLP. All rights reserved.