Antibodies, Proteins, Kits and Reagents for Life Science

241 228 ₸ Product size

100 µl 241 228 ₸

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Anti-GLP1 antibody [EPR4042-1] (ab108443)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4042-1] to GLP1
Suitable for: ELISA, ICC/IF, WB, IHC-P
Reacts with: Human

Product name

Anti-GLP1 antibody [EPR4042-1]
See all GLP1 primary antibodies
Description

Rabbit monoclonal [EPR4042-1] to GLP1
Host species

Rabbit

Tested Applications & Species

Application	Species
ELISA	Recombinant fragment
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Glicentin polypeptide, Human pancreas tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4042-1
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)

Unpurified ab108443, at 1/100 dilution, staining Glicentin in Human pancreas tissue by immunohistochemistry

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)

1 ug/mL of Glucagon-like Peptide (GLP-1) was coated into 96-wells. Various concentrations of anti-Glucagon-Like Peptide 1 (GLP-1, 7-36,amide) rabbit monoclonal antibody (unpurified ab108443) was added. HRP conjugated goat anti-rabbit IgG antibody was used to develop the color.
Immunocytochemistry/ Immunofluorescence - Anti-GLP1 antibody [EPR4042-1] (ab108443)

Immunofluorescence staining of BxPC-3 cells with purified ab108443 at a working dilution of 1/800, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108443 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)

Immunohistochemical staining of paraffin embedded human pancreas with purified ab108443 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-GLP1 antibody [EPR4042-1] (ab108443)

Anti-GLP1 antibody [EPR4042-1] (ab108443) at 1/2000 dilution (purified) + human fetal pancreas lysate at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 21 kDa
Observed band size: 11 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)

Immunohistochemical staining of paraffin embedded human liver with purified ab108443 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)

Antigen dose-response curve using purified ab108443. Antibody concentration of 1 µg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)

Antibody dose-response curve using purified ab108443. Antigen concentration of 1 µg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
Western blot - Anti-GLP1 antibody [EPR4042-1] (ab108443)

Anti-GLP1 antibody [EPR4042-1] (ab108443) at 1/200 dilution (unpurified) + Glicentin polypeptide at 0.005 µg

Predicted band size: 21 kDa
Anti-GLP1 antibody [EPR4042-1] (ab108443)

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