Anti-GLP1 antibody [4F3] (ab23472)
![Anti-GLP1 antibody [4F3] (ab23472)](https://www.abcam.com/ps/products/23/ab23472/Images/ab23472-33331-anti-glp1-antibody-4f3-immunohistochemistry.jpg "Anti-GLP1 antibody [4F3] (ab23472)")
Key features and details
- Mouse monoclonal [4F3] to GLP1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-GLP1 antibody [4F3]
See all GLP1 primary antibodies -
Description
Mouse monoclonal [4F3] to GLP1 -
Host species
Mouse -
Specificity
Reacts with all forms of GLP1, including precursor and GLP1(9-37) /GLP1(9-36amide) metabolite. ab23472 cross-reacts with Liraglutide. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mammals
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Immunogen
Full length protein corresponding to GLP1. Synthetic GLP-1(7-36)amide coupled to PPD
Database link: 2641 -
Epitope
Mid molecular epitope of GLP1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4F3 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [4F3] (ab23472)ab23472 (1µg/ml) staining GLP1 in human pancreas, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
