Starch Assay Kit (ab83393)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell Lysate, Food samples, Tissue Lysate
- Sensitivity: 4 µg/ml
Overview
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Product name
Starch Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell Lysate, Tissue Lysate, Food samples -
Assay type
Quantitative -
Sensitivity
> 4 µg/ml -
Range
4 µg/ml - 2000 µg/ml -
Assay time
0h 40m -
Product overview
Abcam's Starch Assay Kit provides an easy, accurate assay to measure starch levels in a variety of samples. In the assay, starch is hydrolyzed to glucose which is oxidized to generate color (570 nm) and fluorescence (Ex/Em = 535/587 nm). The assay can detect starch at 0.0004 to 2 mg/ml.
Visit our FAQs page for tips and troubleshooting. -
Notes
Starch is a complex carbohydrate consisting of a large number of glucose units. All plants contain starch, present as amylose, (linear α-1,4 linked polymer) and amylopectin, (highly α-1,6 branched α-1,4 polymer). Starch generally contains 0-25% amylose and 75-100% amylopectin.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Development Buffer WM 1 x 25ml Development Enzyme Mix (Lyophilised) Green 1 vial Hydrolysis Buffer NM 1 x 25ml Hydrolysis Enzyme Mix (Lyophilised) Blue 1 vial OxiRed Probe in DMSO Red 1 vial Starch Standard (2.0 mg/ml) Yellow 1 x 100µl -
Research areas
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Relevance
Starch is a complex carbohydrate consisting of a large number of glucose units. All plants contain starch, present as amylose, (linear alpha-1,4 linked polymer) and amylopectin, (highly alpha-1,6 branched alpha-1,4 polymer). Starch generally contains 0-25% amylose and 75–100% amylopectin. -
Alternative names
- Amylopectin
- Amylose
Images
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Functional Studies - Starch Assay Kit (ab83393)Starch Standard Curve: Different types of pure starch were extracted with 10N KOH/H3PO4 as described. Assays were performed following the kit protocol.