Cortisol ELISA Kit (ab108665)
Key features and details
- Sensitivity: 2.44 ng/ml
- Range: 10 ng/ml - 500 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Cortisol ELISA Kit
See all Cortisol kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 60 Inter-assay Sample n Mean SD CV% Overall 30 -
Sample type
Urine, Serum, Hep Plasma, Cit plasma -
Assay type
Competitive -
Sensitivity
= 2.44 ng/ml -
Range
10 ng/ml - 500 ng/ml -
Recovery
103.56 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in serum and plasma.
A 96-well plate has been precoated with anti-Cortisol IgG. Samples and the Cortisol-HRP conjugate are added to the wells, where any Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Washing Solution 1 x 50ml Anti-Cortisol (IgG) Coated Microplate (12 x 8 wells) 1 unit Cortisol Control 1 x 1ml Cortisol Standard 0 – 0 ng/mL 1 x 1ml Cortisol Standard 1 – 10 ng/mL 1 x 1ml Cortisol Standard 2 – 50 ng/mL 1 x 1ml Cortisol Standard 3 – 150 ng/mL 1 x 1ml Cortisol Standard 4 – 500 ng/mL 1 x 1ml Cortisol-HRP conjugate 1 x 21ml Stop Solution 1 x 15ml TMB Substrate Solution 1 x 15ml -
Research areas
Images
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Biological fluids for ab108665
Cortisol measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 1-2 fold.
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Typical Standard CurveRepresentative Standard Curve using ab108665
