Cortisol ELISA Kit (ab154996)
Key features and details
- Sensitivity: 0.12 ng/ml
- Range: 0.5 ng/ml - 100 ng/ml
- Sample type: Saliva
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Cortisol ELISA Kit
See all Cortisol kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Saliva 60 Inter-assay Sample n Mean SD CV% Saliva 30 -
Sample type
Saliva -
Assay type
Competitive -
Sensitivity
0.12 ng/ml -
Range
0.5 ng/ml - 100 ng/ml -
Recovery
95.42 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in saliva.
A 96-well plate has been precoated with anti-Cortisol IgG. Samples and the Cortisol-HRP conjugate are added to the wells, where Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Solution 1 x 50ml Anti-Cortisol IgG Coated Microplate (12 x 8 wells) 1 unit Control A 1 x 1ml Control B 1 x 1ml Cortisol Standard 0 – 0 ng/mL 1 x 1ml Cortisol Standard 1 - 0.5 ng/mL 1 x 1ml Cortisol Standard 2 – 1 ng/mL 1 x 1ml Cortisol Standard 3 – 5 ng/mL 1 x 1ml Cortisol Standard 4 – 10 ng/mL 1 x 1ml Cortisol Standard 5 – 20 ng/mL 1 x 1ml Cortisol Standard 6 – 100 ng/mL 1 x 1ml Cortisol-HRP Conjugate (Concentrated) 1 x 1ml Foil Cover 1 unit Incubation Buffer 1 x 30ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
Research areas
Images
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Typical Standard Curve
Representative Standard Curve using ab154996.
