Anti-NOX1 antibody (ab78016)
Key features and details
- Rabbit polyclonal to NOX1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NOX1 antibody
See all NOX1 primary antibodies -
Description
Rabbit polyclonal to NOX1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from the C terminal of Human NOX1
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Positive control
- Human colon tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX1 antibody (ab78016)ab78016, at 10µg/ml, staining NOX1 in Human colon by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
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Western blot - Anti-NOX1 antibody (ab78016)Anti-NOX1 antibody (ab78016) at 1 µg/ml + Human Prostate Tumor Lysate Tissue Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
NOX1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
