Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20710] to CD226 - BSA and Azide free
  • Suitable for: ICC, WB, IP, IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD226 antibody [EPR20710] - BSA and Azide free
    See all CD226 primary antibodies

  • Description

    Rabbit monoclonal [EPR20710] to CD226 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251537 is the carrier-free version of ab212077. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251537 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)
    Immunocytochemistry - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)
    This data was developed using ab212077, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized EL4.IL-2 (mouse lymphoma T lymphocyte cell line) cells labeling CD226 with ab212077 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)(ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on EL4.IL-2 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
  • Western blot - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)
    Western blot - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)
    All lanes : Anti-CD226 antibody [EPR20710] (ab212077) at 1/1000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : EL4.IL-2 (mouse lymphoma T lymphocyte cell line) whole cell lysate
    Lane 3 : YAC-1 (mouse Moloney murine leukemia virus induced lymphoma lymphoblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 38 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab212077, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 1 minute; Lane 3: 3 minutes.

    The different molecular weights (~65kDa) observed in different tissues or cell lines may be due to different level of CD226 glycosylation (PMID: 8673704).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)
    This data was developed using ab212077, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD226 with ab212077 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining on megakaryocytes and platelets of mouse spleen is observed (PMID: 15693793). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)
    This data was developed using ab212077, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CD226 with ab212077 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Positive staining on platelets of mouse lung is observed (PMID: 15693793). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)
    Immunoprecipitation - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)

    This data was developed using ab212077, the same antibody clone in a different buffer formulation.

    CD226 was immunoprecipitated from 0.35 mg of mouse spleen lysate with ab212077 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab212077 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: Mouse spleen lysate 10 µg (Input).

    Lane 2: ab212077 IP in mouse spleen lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab212077 in mouse spleen lysate

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 5 seconds.

  • Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)
    Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)

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