Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24152-62] to DNAJC5B - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, IP, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free
    See all DNAJC5B primary antibodies

  • Description

    Rabbit monoclonal [EPR24152-62] to DNAJC5B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IP, IHC-Frmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human cerebellum, Mouse brain, Rat brain and His-tagged human DNAJC5/CSP recombinant protein, Myc/DDK-tagged human DNAJC5B recombinant protein lysates. IHC-P: Human cerebrum and Mouse cerebrum, Rat cerebrum tissues. IHC-Fr: Mouse cerebral cortex, Rat cerebral cortex tissues. Flow Cyt: Mouse primary neuron cell. IP: Human cerebellum tissue lysates.

  • General notes

    ab281840 is the carrier-free version of ab259867. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab281840 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24152-62

  • Isotype

    IgG

Images

  • Western blot - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)
    Western blot - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)
    All lanes : Anti-DNAJC5B antibody [EPR24152-62] (ab259867) at 1/1000 dilution

    Lane 1 : Human cerebellum tissue lysate
    Lane 2 : Human kidney tissue lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Mouse kidney tissue lysate
    Lane 5 : Mouse testis tissue lysate
    Lane 6 : Rat brain tissue lysate
    Lane 7 : Rat kidney tissue lysate
    Lane 8 : Rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 23 kDa
    Observed band size: 22-30 kDa why is the actual band size different from the predicted?



    This data was developed using ab259867, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    DNAJC5A has non-palmitoylated and palmitoylated forms as described in the literature (PMID: 32042150).

    Low expression: kidney (PMID: 20499929).

     Exposure time: 26 seconds

     

  • Western blot - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)
    Western blot - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)
    All lanes : Anti-DNAJC5B antibody [EPR24152-62] (ab259867) at 1/1000 dilution

    Lane 1 : His-tagged human DNAJC5/CSP recombinant protein (aa 2-100) at 0.01 µg
    Lane 2 : Myc/DDK-tagged human DNAJC5B recombinant protein (aa 1-199) at 0.02 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 23 kDa



    This data was developed using ab259867, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This antibody reacts with both DNAJC5A and DNAJC5B.

    Exposure time: 37 seconds

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)

    This data was developed using ab259867, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling DNAJC5A+5B with ab259867 at 1/300 (558.0 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cerebrum. The section was incubated with ab259867 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)

    This data was developed using ab259867, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling DNAJC5A+5B with ab259867 at 1/300 (558.0 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab259867 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)

    This data was developed using ab259867, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling DNAJC5A+5B with ab259867 at 1/300 (558.0 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebrum. The section was incubated with ab259867 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)
    Immunohistochemistry (Frozen sections) - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)

    This data was developed using ab259867, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebral cortex tissue labeling DNAJC5A+5B with ab259867 at 1/100 (5.58 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Cytoplasmic staining on mouse cerebral cortex is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)
    Immunohistochemistry (Frozen sections) - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)

    This data was developed using ab259867, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebral cortex tissue labeling DNAJC5A+5B with ab259867 at 1/100 (5.58 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Cytoplasmic staining on rat cerebral cortex is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Flow Cytometry - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)
    Flow Cytometry - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)

    This data was developed using ab259867, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of Mouse primary neuron cells cells labelling DNAJC5A+5B with ab259867 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Immunoprecipitation - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)
    Immunoprecipitation - Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)

    This data was developed using ab259867, the same antibody clone in a different buffer formulation.

    DNAJC5A+5B was immunoprecipitated from 0.35 mg Human cerebellum tissue lysate 10 ug with ab259867 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259867 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human cerebellum tissue lysate 10 ug

    Lane 2: ab259867 IP in Human cerebellum tissue lysate

    Lane 3: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated :Rabbit monoclonal IgG (ab172730) instead of ab259867 in human cerebellum tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 7.5 seconds

     

  • Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)
    Anti-DNAJC5B antibody [EPR24152-62] - BSA and Azide free (ab281840)

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