Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µg 291 484 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

Key features and details

Mouse monoclonal [RGE53] to Cytokeratin 18
Suitable for: Flow Cyt, IHC-Fr, WB
Reacts with: Human, Pig, Zebrafish
Isotype: IgG1

Product name

Anti-Cytokeratin 18 antibody [RGE53]
See all Cytokeratin 18 primary antibodies
Description

Mouse monoclonal [RGE53] to Cytokeratin 18
Host species

Mouse
Specificity

This antibody reacts exclusively with cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-Fr	Human Pig Zebrafish
WB	Human

See all applications and species data

Immunogen

Cytoskeletal preparation of cells
Positive control
- This antibody gave a positive signal in HeLa and MCF7 whole cell lysates.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

RGE53
Myeloma

unknown
Isotype

IgG1
Research areas

Western blot - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

All lanes : Anti-Cytokeratin 18 antibody [RGE53] (ab9217) at 5 µg/ml

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 48 kDa
Observed band size: 48 kDa

Exposure time: 30 seconds
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

Immunohistochemistry on frozen section of human kidney epithelium.
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

Immunofluorescence staining images of 9 day old zebrafish embryos.
ab9217 reacts with with intestinal epithelium and epidermis. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.0
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

immunofluorescence staining of epithelial tissues in a 2 days old zebrafish embryo.
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

Immunofluorescence staining of 1 month old zebrafish embryo.
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

Immunofluorescence staining images of 9 day old zebrafish embryos.
ab9217 reacts with intestinal epithelium and epidermis. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

Immunohistochemistry on frozen section of human kidney epithelium
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

Immunohistochemistry on frozen section of human colon
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

Immunohistochemistry on frozen section of swine liver hepatocytes
Flow Cytometry - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

Overlay histogram showing MCF7 cells stained with ab9217 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9217, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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