Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
Key features and details
- Mouse monoclonal [RGE53] to Cytokeratin 18
- Suitable for: Flow Cyt, IHC-Fr, WB
- Reacts with: Human, Pig, Zebrafish
- Isotype: IgG1
Overview
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Product name
Anti-Cytokeratin 18 antibody [RGE53]
See all Cytokeratin 18 primary antibodies -
Description
Mouse monoclonal [RGE53] to Cytokeratin 18 -
Host species
Mouse -
Specificity
This antibody reacts exclusively with cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-Fr HumanPigZebrafishWB Human -
Immunogen
Cytoskeletal preparation of cells
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Positive control
- This antibody gave a positive signal in HeLa and MCF7 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
RGE53 -
Myeloma
unknown -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-Cytokeratin 18 antibody [RGE53] (ab9217) at 5 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 30 seconds
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Immunohistochemistry on frozen section of human kidney epithelium.
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Immunofluorescence staining images of 9 day old zebrafish embryos.
ab9217 reacts with with intestinal epithelium and epidermis. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.0 -
immunofluorescence staining of epithelial tissues in a 2 days old zebrafish embryo.
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Immunofluorescence staining of 1 month old zebrafish embryo.
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Immunofluorescence staining images of 9 day old zebrafish embryos.
ab9217 reacts with intestinal epithelium and epidermis. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature. -
Immunohistochemistry on frozen section of human kidney epithelium
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Immunohistochemistry on frozen section of human colon
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Immunohistochemistry on frozen section of swine liver hepatocytes
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Overlay histogram showing MCF7 cells stained with ab9217 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9217, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.