Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)] (ab192877)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7044(2)] to MIP-3 beta/CCL19
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)]
See all MIP-3 beta/CCL19 primary antibodies -
Description
Rabbit monoclonal [EPR7044(2)] to MIP-3 beta/CCL19 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A549 cells; Human MIP-3 beta/CCL19 recombinant protein (aa22-98).
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General notes
This product was previously labelled as Macrophage Inflammatory Protein 3 beta
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7044(2) -
Isotype
IgG -
Research areas
Images
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Flow cytometric analysis of 2% paraformaldehyde-fixed A549 cells labeling MIP-3 beta/CCL19 with ab192877 at 1/170 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)] (ab192877) at 1/20000 dilution + Human MIP-3 beta/CCL19 recombinant protein (aa22-98) at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
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