Mouse alpha 1 acid Glycoprotein ELISA Kit (ab264605)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 111.4 pg/ml
- Range: 156.25 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse alpha 1 acid Glycoprotein ELISA Kit
See all Alpha 1 Acid Glycoprotein/AGP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.2% Inter-assay Sample n Mean SD CV% Serum 3 3.4% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
111.4 pg/ml -
Range
156.25 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 103 98% - 112% Serum 104 99% - 108% Hep Plasma 108 100% - 116% EDTA Plasma 111 108% - 114% Cit plasma 103 94% - 112% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse alpha 1 acid Glycoprotein ELISA Kit (ab264605) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of alpha 1 acid Glycoprotein protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse alpha 1 acid Glycoprotein with 111.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Alpha 1 acid glycoprotein 1 (ORM1) is a plasma protein involved in acute inflammation. Alpha 1 acid glycoprotein is a transport protein for various cellular ligands and synthetic drugs. Alpha 1 acid glycoprotein is expressed by the liver and is induced by glucocorticoids, IL-1 and IL-6.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse alpha 1 acid Glycoprotein Capture Antibody 1 x 600µl 10X Mouse alpha 1 acid Glycoprotein Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse alpha 1 acid Glycoprotein Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Appears to function in modulating the activity of the immune system during the acute-phase reaction. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- A1AG1_HUMAN
- AGP
- AGP 1
see all -
Database links
- Entrez Gene: 18405 Mouse
- SwissProt: Q60590 Mouse
- Unigene: 4777 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse alpha 1 acid Glycoprotein standard curve in Sample Diluent NS.The alpha 1 acid Glycoprotein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native alpha 1 acid Glycoprotein in mouse serum and plasma samples.The concentrations of alpha 1 acid Glycoprotein were measured in duplicates, interpolated from the alpha 1 acid Glycoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 2,000, plasma (citrate) 1: 8,000, plasma (heparin) 1: 4,000, and plasma (EDTA) 1: 2,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 acid Glycoprotein concentration was determined to be 16.84 µg/mL in serum, 47.40 µg/mL in plasma (citrate), 24.08 µg/ml in plasma (heparin), and 17.47 µg/ml in plasma (EDTA).
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Interpolated concentrations of native alpha 1 acid Glycoprotein in mouse liver tissue culture supernatant samples.The concentrations of alpha 1 acid Glycoprotein were measured in duplicates, interpolated from the alpha 1 acid Glycoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows: mouse liver supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 acid Glycoprotein concentration was determined to be 10.66 ng/mL in liver supernatant.
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Interpolated concentrations of native alpha 1 acid Glycoprotein in male mouse urine samples.The concentrations of alpha 1 acid Glycoprotein were measured in duplicates, interpolated from the alpha 1 acid Glycoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows: male mouse urine 1: 256. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 acid Glycoprotein concentration was determined to be 1.94 µg/mL in male mouse urine.
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Sandwich ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit (ab264605)To learn more about the advantages of recombinant antibodies see here.
