Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

Order now and get it on Wednesday March 10, 2021

Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23610-40] to CCL4/MIP-1 beta
Suitable for: ICC, Flow Cyt, Dot blot
Reacts with: Mouse, Human

Product name

Anti-CCL4/MIP-1 beta antibody [EPR23610-40]
See all CCL4/MIP-1 beta primary antibodies
Description

Rabbit monoclonal [EPR23610-40] to CCL4/MIP-1 beta
Host species

Rabbit
Specificity

This antibody has cross-reactivity with CCL4L1. There is about 97% homology between CCL4/MIP-1 beta and CCL4L1 in terms of the immunogen designed from CCL4/MIP-1 beta.

Tested Applications & Species

Application	Species
Dot	Human
Flow Cyt	Mouse Human
ICC	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- ICC: THP-1 cells (treated with PMA, 100nM for 16 hours, then with lipopolysaccharide (LPS, 100 ng/ml) for 4 hours and with BFA (1 µg/ml) for another 3 hours.). Flow cyt: THP-1 (treated with 100nM phorbol 12-myristate 13-acetate (PMA) for 16 hours, then 100 ng/ml lipopolysaccharide (LPS) for 4 hours and add 1 ug/ml Brefeldin A (BFA) for another 3 hours) and RAW 264.7 cells (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours and 1 ug/ml Brefeldin A (BFA) for another 3 hours).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23610-40
Isotype

IgG
Research areas

Flow Cytometry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) (treated with 100nM phorbol 12-myristate 13-acetate (PMA) for 16 hours, then 100 ng/ml lipopolysaccharide (LPS) for 4 hours and add 1 ug/ml Brefeldin A (BFA) for another 3 hours) (Red)/Untreated control (Green) cells labelling CCL4/MIP-1 beta with ab254371 at 1/500 dilution (0.1ug)/ compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti-rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

The expression pattern is consistent with what is described in the literature (PMID:29669317).
Immunocytochemistry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 cells labelling CCL4/MIP-1 beta with ab254371 at 1/100 (5.45 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in THP-1 cells treated with phorbol 12-myristate 13-acetate (PMA, 100nM) for 16 hours, then with lipopolysaccharide (LPS, 100 ng/ml) for 4 hours and with BFA (1 ug/ml) for another 3 hours. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Dot Blot - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)

Dot blot analysis of CCL4/MIP-1 beta using ab254371 at 1/1000 (0.545ug/ml) dilution followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20,000 dilution.

Lane 1: Human CCL4/MIP-1 beta immunogen recombinant protein

Lane 2: Human CCL4L1 recombinant protein

Lane 3: Bovine serum albumin (BSA)

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 114 seconds
Flow Cytometry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours and 1 ug/ml Brefeldin A (BFA) for another 3 hours) (Red) /Untreated control (Green) cells labelling CCL4/MIP-1 beta with ab254371 at 1/50 dilution (1ug)/ compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti-rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

The expression pattern is consistent with what is described in the literature (PMID:32230927).
Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)

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