Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23610-40] to CCL4/MIP-1 beta
  • Suitable for: ICC, Flow Cyt, Dot blot
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CCL4/MIP-1 beta antibody [EPR23610-40]
    See all CCL4/MIP-1 beta primary antibodies

  • Description

    Rabbit monoclonal [EPR23610-40] to CCL4/MIP-1 beta

  • Host species

    Rabbit

  • Specificity

    This antibody has cross-reactivity with CCL4L1. There is about 97% homology between CCL4/MIP-1 beta and CCL4L1 in terms of the immunogen designed from CCL4/MIP-1 beta.

  • Tested Applications & Species

    Application Species
    Dot
    Human
    Flow Cyt
    Mouse
    Human
    ICC
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: THP-1 cells (treated with PMA, 100nM for 16 hours, then with lipopolysaccharide (LPS, 100 ng/ml) for 4 hours and with BFA (1 µg/ml) for another 3 hours.). Flow cyt: THP-1 (treated with 100nM phorbol 12-myristate 13-acetate (PMA) for 16 hours, then 100 ng/ml lipopolysaccharide (LPS) for 4 hours and add 1 ug/ml Brefeldin A (BFA) for another 3 hours) and RAW 264.7 cells (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours and 1 ug/ml Brefeldin A (BFA) for another 3 hours).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)
    Flow Cytometry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) (treated with 100nM phorbol 12-myristate 13-acetate (PMA) for 16 hours, then 100 ng/ml lipopolysaccharide (LPS) for 4 hours and add 1 ug/ml Brefeldin A (BFA) for another 3 hours) (Red)/Untreated control (Green) cells labelling CCL4/MIP-1 beta with ab254371 at 1/500 dilution (0.1ug)/ compared with a Rabbit monoclonal IgG (ab172730)  isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    The expression pattern is consistent with what is described in the literature (PMID:29669317).

  • Immunocytochemistry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)
    Immunocytochemistry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 cells labelling CCL4/MIP-1 beta with ab254371 at 1/100 (5.45 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in THP-1 cells treated with phorbol 12-myristate 13-acetate (PMA, 100nM) for 16 hours, then with lipopolysaccharide (LPS, 100 ng/ml) for 4 hours and with BFA (1 ug/ml) for another 3 hours. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Dot Blot - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)
    Dot Blot - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)

    Dot blot analysis of CCL4/MIP-1 beta using ab254371 at 1/1000 (0.545ug/ml) dilution followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20,000 dilution.

    Lane 1: Human CCL4/MIP-1 beta immunogen recombinant protein

    Lane 2: Human CCL4L1 recombinant protein

    Lane 3:  Bovine serum albumin (BSA)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 114 seconds

  • Flow Cytometry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)
    Flow Cytometry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours and 1 ug/ml Brefeldin A (BFA) for another 3 hours) (Red) /Untreated control (Green) cells labelling CCL4/MIP-1 beta with ab254371 at 1/50 dilution (1ug)/ compared with a Rabbit monoclonal IgG (ab172730)  isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    The expression pattern is consistent with what is described in the literature (PMID:32230927).

  • Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)
    Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)

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