Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Thursday March 04, 2021

Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5401(2)] to GABA A Receptor alpha 1
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)]
See all GABA A Receptor alpha 1 primary antibodies
Description

Rabbit monoclonal [EPR5401(2)] to GABA A Receptor alpha 1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human GABA A Receptor alpha 1 aa 1-100. The exact sequence is proprietary.
Positive control
- WB: Human fetal brain, Human lung and Human cerebellum lysates; Human brain prepared in 1%SDS Hot lysis method. IHC-P: Human brain, colon, kidney, Ovarian carcinoma, spleen, cerebrum and glioma tissues.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR5401(2)
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling GABA A Receptor alpha 1 with Purified ab151573 at 1:1000 dilution (0.7 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 1/1000 dilution

Lane 1 : Human brain prepared in RIPA lysis method
Lane 2 : Human brain prepared in 1%SDS Hot lysis method

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 52 kDa
Observed band size: 52 kDa

Exposure time: 40 seconds

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 0.7 µg/ml (purified)

Lane 1 : Human brain lysates
Lane 2 : Human lung lysates - negative control

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 52 kDa

Blocking and diluting buffer: 5% NFDM/TBST.

Please note: Lysates prepared in 1%SDS Hot lysis method.

According to the paper (PMID: 29467616), brain is positive and lung is negative tissue.
Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 1/1000 dilution (unpurified)

Lane 1 : Human fetal brain lysate
Lane 2 : Human cerebellum lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 52 kDa

Please note: Lysates prepared in 1%SDS Hot lysis method.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling GABA A Receptor alpha 1 with unpurified ab151573 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling GABA A Receptor alpha 1 with unpurified ab151573 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

Immunohistochemical analysis of paraffin embedded normal Human colon tissue using unpurified ab151573 showing -ve staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using unpurified ab151573 showing -ve staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using unpurified ab151573 showing -ve staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using unpurified ab151573 showing -ve staining.
Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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