Anti-GABA A Receptor alpha 1 antibody (ab33299)
Key features and details
- Rabbit polyclonal to GABA A Receptor alpha 1
- Suitable for: IHC-P, WB, IP
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-GABA A Receptor alpha 1 antibody
See all GABA A Receptor alpha 1 primary antibodies -
Description
Rabbit polyclonal to GABA A Receptor alpha 1 -
Host species
Rabbit -
Specificity
This antibody is specific for the GABA A Receptor a1 subunit. -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Cow, Cynomolgus monkey
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Immunogen
Synthetic peptide:
QPSQDELKDNTTVFT-C
, corresponding to N terminal amino acids 1-15 of the mature Rat GABA A Receptor alpha 1 containing a cysteine at the C terminus for linkage to a carrier protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GABA A Receptor alpha 1 antibody (ab33299)Anti-GABA A Receptor alpha 1 antibody (ab33299) at 1/10000 dilution + rat brain microsomal preparation tissue lysate at 10 µg
Predicted band size: 52 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody (ab33299)Immunohistochemistry analysis of formalin fixed paraffin embedded rat hippocampus tissue prepared using heat-induced epitope retrieval (HIER) labeling GABA A Receptor alpha 1 using ab33299 at 1/500 dilution. Secondary antibody was an anti-rabbit antibody and HRP-DAB.
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Immunoprecipitation - Anti-GABA A Receptor alpha 1 antibody (ab33299)Immunoprecipitation analysis: 1 mg of RIPA lysate of rat brain microsomes
was immunoprecipitated with 5 uL of ab33299. Arrow indicates GABAA
Receptor a1 (~50kD).
