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Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)

Key features and details

  • Mouse monoclonal [N95/35] to GABA A Receptor alpha 1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-GABA A Receptor alpha 1 antibody [N95/35]
    See all GABA A Receptor alpha 1 primary antibodies

  • Description

    Mouse monoclonal [N95/35] to GABA A Receptor alpha 1

  • Host species

    Mouse

  • Specificity

    ab94585 does not react with GABA A Receptor Alpha 2 or Alpha 3.

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Fusion protein corresponding to Mouse GABA A Receptor alpha 1 aa 355-394.
    Sequence:

    KVKDPLIKKNNTYAPTATSYTPNLARGDPGLATIAKSATI


    Database link: P62812
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Rat brain normal tissue lysate - membrane extract (ab29473), Human cell line mix lysate. IHC-P: Mouse back skin tissue.

  • General notes

    The clone number has been updated from S95-35 to N95/35, both clone numbers name the same antibody clone.

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)

    Bouin's Fixative and paraffin-embedded mouse back skin tissue stained for GABA A Receptor alpha 1 with ab94585 (1/100 dilution for 1 hour at RT) in immunohistochemical analysis.

    Secondary antibody is a FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal Cells.

  • Western blot - Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)
    Western blot - Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)
    Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585) at 1/1000 dilution + Human cell line mix lysates at 15 µg

    Secondary
    Sheep Anti-Mouse IgG: HRP

    Predicted band size: 52 kDa



    Block: 1% BSA.

    Primary antibody incubated for 2 hours at RT.

    Secondary antibody incubated for 1 hour at RT.

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