Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)
![Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)](https://www.abcam.com/ps/products/94/ab94585/Images/ab94585-311029-anti-gaba-a-receptor-alpha-1-antibody-n95-35-immunohistochemistry.jpg "Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)")
Key features and details
- Mouse monoclonal [N95/35] to GABA A Receptor alpha 1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-GABA A Receptor alpha 1 antibody [N95/35]
See all GABA A Receptor alpha 1 primary antibodies -
Description
Mouse monoclonal [N95/35] to GABA A Receptor alpha 1 -
Host species
Mouse -
Specificity
ab94585 does not react with GABA A Receptor Alpha 2 or Alpha 3. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB Human
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Immunogen
Fusion protein corresponding to Mouse GABA A Receptor alpha 1 aa 355-394.
Sequence:KVKDPLIKKNNTYAPTATSYTPNLARGDPGLATIAKSATI
Database link: P62812 -
Positive control
- WB: Rat brain normal tissue lysate - membrane extract (ab29473), Human cell line mix lysate. IHC-P: Mouse back skin tissue.
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General notes
The clone number has been updated from S95-35 to N95/35, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N95/35 -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)
Bouin's Fixative and paraffin-embedded mouse back skin tissue stained for GABA A Receptor alpha 1 with ab94585 (1/100 dilution for 1 hour at RT) in immunohistochemical analysis.
Secondary antibody is a FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal Cells.
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![Western blot - Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)](https://www.abcam.com/ps/products/94/ab94585/Images/ab94585-199674-anti-gaba-a-receptor-alpha-1-antibody-n95-35-western-blot.jpg)
Western blot - Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585) at 1/1000 dilution + Human cell line mix lysates at 15 µg
Secondary
Sheep Anti-Mouse IgG: HRP
Predicted band size: 52 kDaBlock: 1% BSA.
Primary antibody incubated for 2 hours at RT.
Secondary antibody incubated for 1 hour at RT.
