Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ABAT/GABA-T antibody [EPR4433] - BSA and Azide free (ab232525)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4433] to ABAT/GABA-T - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ABAT/GABA-T antibody [EPR4433] - BSA and Azide free
    See all ABAT/GABA-T primary antibodies

  • Description

    Rabbit monoclonal [EPR4433] to ABAT/GABA-T - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Mouse cross reactivity tested by western blot and IHC. Rat cross reactivity tested by western blot only.

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human liver tissue.

  • General notes

    ab232525 is the carrier-free version of ab108249 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab232525 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as ABAT.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-ABAT/GABA-T antibody [EPR4433] - BSA and Azide free (ab232525)
    Immunoprecipitation - Anti-ABAT/GABA-T antibody [EPR4433] - BSA and Azide free (ab232525)

    ab108249 (purified) at 1:40 dilution (2µg) immunoprecipitating ABAT/GABA-T in HepG2 whole cell lysate.
    Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab108249 & HepG2 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108249 in HepG2 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108249).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] - BSA and Azide free (ab232525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] - BSA and Azide free (ab232525)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling ABAT/GABA-T with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108249).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] - BSA and Azide free (ab232525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] - BSA and Azide free (ab232525)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling ABAT/GABA-T with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108249).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] - BSA and Azide free (ab232525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] - BSA and Azide free (ab232525)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling ABAT/GABA-T with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108249).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] - BSA and Azide free (ab232525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] - BSA and Azide free (ab232525)

    Immunohistocemical analysis of human liver tissue labeling ABAT/GABA-T using ab108249 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108249).

  • Anti-ABAT/GABA-T antibody [EPR4433] - BSA and Azide free (ab232525)
    Anti-ABAT/GABA-T antibody [EPR4433] - BSA and Azide free (ab232525)

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