Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SUFU antibody [EPR23821-101] - BSA and Azide free (ab275747)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23821-101] to SUFU - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SUFU antibody [EPR23821-101] - BSA and Azide free
    See all SUFU primary antibodies

  • Description

    Rabbit monoclonal [EPR23821-101] to SUFU - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC,IHC-Fr,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, HEK-293T, NIH/3T3, PC-12 and LNCaP whole cell lysates. Flow cyt: HAP1 cells.

  • General notes

    ab275747 is the carrier-free version of ab259975. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275747 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-SUFU antibody [EPR23821-101] - BSA and Azide free (ab275747)
    Western blot - Anti-SUFU antibody [EPR23821-101] - BSA and Azide free (ab275747)
    All lanes : Anti-SUFU antibody [EPR23821-101] (ab259975) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell), whole cell lysate
    Lane 2 : SUFU knockout HAP1 (human chronic myelogenous leukemia near-haploid cell), whole cell lysate
    Lane 3 : Wild-type HEK293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 4 : SUFU knockout HEK293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 5 : LNCaP (human prostate carcinoma epithelial cell), whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at 1/10000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    This data was developed using ab259975, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lanes 1-5: Merged signal (red and green). Green - ab259975 observed at 53 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

    Lanes 1-2: ab253183 Anti-SUFU antibody [EPR23821-101] was shown to react with SUFU in HAP1 cells in Western blot. Loss of signal was observed when SUFU knockout sample was used. Wild-type and SUFU knockout samples were subjected to SDS-PAGE.

     Lanes 3-4: ab253183 Anti-SUFU antibody [EPR23821-101] was shown to specifically react with SUFU in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267282 (knockout cell lysate ab257718) was used.  Wild-type and SUFU knockout samples were subjected to SDS-PAGE. 

    ab253183 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4° overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

  • Flow Cytometry - Anti-SUFU antibody [EPR23821-101] - BSA and Azide free (ab275747)
    Flow Cytometry - Anti-SUFU antibody [EPR23821-101] - BSA and Azide free (ab275747)

    This data was developed using ab259975, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized parental HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line, Right) / SUFU KO HAP1 (Left) cells labelling SUFU with ab259975 at 1/500 dilution (0.1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730)  isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-SUFU antibody [EPR23821-101] - BSA and Azide free (ab275747)
    Western blot - Anti-SUFU antibody [EPR23821-101] - BSA and Azide free (ab275747)
    All lanes : Anti-SUFU antibody [EPR23821-101] (ab259975) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : 293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    This data was developed using ab259975, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Fresh lysates were used in this WB.

    Exposure time: 26 seconds.

  • Western blot - Anti-SUFU antibody [EPR23821-101] - BSA and Azide free (ab275747)
    Western blot - Anti-SUFU antibody [EPR23821-101] - BSA and Azide free (ab275747)
    Anti-SUFU antibody [EPR23821-101] (ab259975) at 1/1000 dilution + LNCaP (human prostate carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    This data was developed using ab259975, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Fresh lysates were used in this WB.

    Exposure time: 37 seconds.

     

  • Anti-SUFU antibody [EPR23821-101] - BSA and Azide free (ab275747)
    Anti-SUFU antibody [EPR23821-101] - BSA and Azide free (ab275747)

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