Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4433] to ABAT/GABA-T
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ABAT/GABA-T antibody [EPR4433]
    See all ABAT/GABA-T primary antibodies

  • Description

    Rabbit monoclonal [EPR4433] to ABAT/GABA-T

  • Host species

    Rabbit

  • Specificity

    Mouse cross reactivity tested by western blot and IHC. Rat cross reactivity tested by western blot only.

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2 cell lysate, SH-SY5Y cell lysae, Human fetal liver tissue lysate, rat brain tissue lysate, rat kidney tissue lysate, mouse brain tissue lysate, Human liver tissue.

  • General notes

     

     

    Previously labelled as ABAT. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)
    Western blot - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)
    All lanes : Anti-ABAT/GABA-T antibody [EPR4433] (ab108249) at 1/10000 dilution (Purified)

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates with 5% NFDM /TBST
    Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates with 5% NFDM /TBST
    Lane 3 : Mouse brain lysates with 5% NFDM /TBST
    Lane 4 : Rat brain lysates with 5% NFDM /TBST

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 56 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling ABAT/GABA-T with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunoprecipitation - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)
    Immunoprecipitation - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)

    ab108249 (purified) at 1:40 dilution (2µg) immunoprecipitating ABAT/GABA-T in HepG2 whole cell lysate.
    Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab108249 & HepG2 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108249 in HepG2 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling ABAT/GABA-T with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling ABAT/GABA-T with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)

    Unpurified ab108249, at 1/250 dilution, staining ABAT/GABA-T in Human liver tissue by immunohistochemistry.

  • Western blot - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)
    Western blot - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)
    All lanes : Anti-ABAT/GABA-T antibody [EPR4433] (ab108249) (unpurified)

    Lane 1 : HepG2 cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : Human fetal liver tissue lysate
    Lane 4 : rat brain tissue lysate
    Lane 5 : rat kidney tissue lysate
    Lane 6 : mouse brain tissue lysate

    Predicted band size: 56 kDa

  • Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)
    Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)

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