Anti-NDUFC2 antibody [EPR16499] (ab192265)
![Anti-NDUFC2 antibody [EPR16499] (ab192265)](https://www.abcam.com/ps/products/192/ab192265/Images/ab192265-230107-anti-ndufc2-antibody-epr16499-western-blot.jpg "Anti-NDUFC2 antibody [EPR16499] (ab192265)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16499] to NDUFC2
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-NDUFC2 antibody [EPR16499]
See all NDUFC2 primary antibodies -
Description
Rabbit monoclonal [EPR16499] to NDUFC2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, HepG2 and A549 cell lysates; Human kidney tissue; A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16499 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NDUFC2 antibody [EPR16499] (ab192265)All lanes : Anti-NDUFC2 antibody [EPR16499] (ab192265) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFC2 antibody [EPR16499] (ab192265)](https://www.abcam.com/ps/products/192/ab192265/Images/ab192265-230108-anti-ndufc2-antibody-epr16499-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFC2 antibody [EPR16499] (ab192265)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFC2 with ab192265 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-NDUFC2 antibody [EPR16499] (ab192265)](https://www.abcam.com/ps/products/192/ab192265/Images/ab192265-230109-anti-ndufc2-antibody-epr16499-flow-cytometry.jpg)
Flow Cytometry - Anti-NDUFC2 antibody [EPR16499] (ab192265)Flow cytometric analysis of 2% paraformaldehyde-fixed A549 cells labeling NDUFC2 with ab192265 at 1/30 dilution (pink) compared with a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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![Anti-NDUFC2 antibody [EPR16499] (ab192265)](https://www.abcam.com/ps/products/192/ab192265/Images/ab192265-4-benefits-of-recombinant-antibodies.png)
Anti-NDUFC2 antibody [EPR16499] (ab192265)
