Anti-GABA antibody [5A9] (ab86186)
Key features and details
- Mouse monoclonal [5A9] to GABA
- Suitable for: IHC-P, ICC/IF, IHC-FoFr
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-GABA antibody [5A9]
See all GABA primary antibodies -
Description
Mouse monoclonal [5A9] to GABA -
Host species
Mouse -
Specificity
ab86186 is highly specific to GABA and does not react with other amino acid BSA conjugates with the exception of weak reaction against beta alanine BSA conjugate at the highest concentrations used. -
Tested applications
Suitable for: IHC-P, ICC/IF, IHC-FoFrmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
GABA coupled to BSA with glutaraldehyde
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General notes
ab86186 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
ab86186 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. -
Clonality
Monoclonal -
Clone number
5A9 -
Isotype
IgG1 -
Research areas
Images
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ab86186 staining GABA in the Brains of 2-5 days old moths (Manduca) by ICC/IF (Immunocytochemistry/immunofluorescence). The head capsules were fixed with 4% paraformaldehyde, washed in PBS and embedded in Low melting point Agarose and section to 75 μm thickness.Section were blocked with 2% BSA(IgG-free) . Samples were incubated with primary antibody (1/500 in PBS).
Double labeling against the Ms5HT1A receptor (green) and GABA (magenta) revealed a subset of Ms5HT1A-ir cell bodies co-localizing GABA (arrows). Scale bar = 100 μm.
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Immunohistochemical analysis of human brain tissue labeling GABA with ab86186 at 1:20 dilution. Sample was incubated with primary antibody for 60 minutes.