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Neuroscience Neurotransmitter Transporters GABA

Anti-GABA antibody [5A9] (ab86186)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-GABA antibody [5A9] (ab86186)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [5A9] to GABA
  • Suitable for: IHC-P, ICC/IF, IHC-FoFr
  • Reacts with: Species independent
  • Isotype: IgG1

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Overview

  • Product name

    Anti-GABA antibody [5A9]
    See all GABA primary antibodies
  • Description

    Mouse monoclonal [5A9] to GABA
  • Host species

    Mouse
  • Specificity

    ab86186 is highly specific to GABA and does not react with other amino acid BSA conjugates with the exception of weak reaction against beta alanine BSA conjugate at the highest concentrations used.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    GABA coupled to BSA with glutaraldehyde

  • General notes


    ab86186 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Primary antibody notes

    ab86186 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
  • Clonality

    Monoclonal
  • Clone number

    5A9
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • GABA

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GABA antibody [5A9] (ab86186)
    Immunocytochemistry/ Immunofluorescence - Anti-GABA antibody [5A9] (ab86186) Image from Dacks AM et al., PLoS One. 2013;8(7):e69422. Fig 5-F.; doi: 10.1371/journal.pone.0069422.

    ab86186 staining GABA in the Brains of 2-5 days old moths (Manduca) by ICC/IF (Immunocytochemistry/immunofluorescence). The head capsules were fixed with 4% paraformaldehyde, washed in PBS and embedded in Low melting point Agarose and section to 75 μm thickness.Section were blocked with 2% BSA(IgG-free) . Samples were incubated with primary antibody (1/500 in PBS).

    Double labeling against the Ms5HT1A receptor (green) and GABA (magenta) revealed a subset of Ms5HT1A-ir cell bodies co-localizing GABA (arrows). Scale bar = 100 μm.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [5A9] (ab86186)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [5A9] (ab86186)

    Immunohistochemical analysis of human brain tissue labeling GABA with ab86186 at 1:20 dilution. Sample was incubated with primary antibody for 60 minutes.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-GABA antibody [5A9] (ab86186)

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    Anti-GABA antibody (ab17413)

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    Applications: IHC-FoFr, IHC-Fr, IM

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    Applications: ICC

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    Anti-GABA antibody (ab8891)

    Applications: ICC/IF, IHC-P

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