Mouse L-Selectin ELISA Kit (CD62L) (ab199085)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.07 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse L-Selectin ELISA Kit (CD62L)
See all CD62L kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 7.56% Inter-assay Sample n Mean SD CV% Serum 3 6.55% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.07 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 96 95% - 97% Cell culture media 96 93% - 102% Cit plasma 97 95% - 99% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Goat, Cow, Pig -
Product overview
Mouse L-Selectin ELISA Kit (CD62L) (ab199085) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of L-Selectin (CD62L) protein in cell culture supernatant, cit plasma, serum, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse L-Selectin (CD62L) with 1.07 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
L-Selectin is a cell surface glycoprotein and a member of a family of adhesion proteins. L-Selectin was first identified in mice as the Mel-14 antigen, a lymph node homing receptor also found on neutrophils and monocytes expressed on a variety of leukocytes. This molecule acts as adhesion and homing receptor that plays an important role in lymphocyte-endothelial cell recognition and migration. The molecule itself is composed of multiple domains: 1 homologous to lectins, 1 to epidermal growth factor, and 2 to the consensus repeat units found in C3/C4 binding proteins.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse L-Selectin Capture Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5B 1 x 6ml Mouse L-Selectin Detector Antibody (lyophilized) 1 vial Mouse L-Selectin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
CD62L is a cell surface component that is a member of a family of adhesion/homing receptors. These play important roles in leukocyte-endothelial cell interactions. CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins. -
Cellular localization
Cell Membrane; single-pass type I membrane protein. -
Alternative names
- A.11
- AI528707
- CD62 antigen ligand
see all -
Database links
- Entrez Gene: 20343 Mouse
- SwissProt: P18337 Mouse
Images
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Other - Mouse L-Selectin ELISA Kit (CD62L) (ab199085)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of L-Selectin standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Example of L-Selectin standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Demonstration of linearity of dilution of the assay.Titration of mouse serum and citrate plasma within the working range of the assay. The 1X dilution for mouse serum and plasma was 0.1% matrix. The interpolated L-Selectin concentrations multiplied by the total dilution factor are shown for each matrix (mean +/- SD, n = 2).
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Titration of stimulated and unstimulated splenocyte supernatant within the working range of the assay.The 1X dilution for both the stimulated and unstimulated supernatants was 100% matrix. The interpolated L-Selectin concentrations multiplied by the total dilution factor are shown for each matrix (mean +/- SD, n = 2).
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Sandwich ELISA - Mouse L-Selectin ELISA Kit (CD62L) (ab199085)To learn more about the advantages of recombinant antibodies see here.
