Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)
![Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)](https://www.abcam.com/ps/products/80/ab80299/Images/ab80299-3-ab80299WB1.jpg "Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)")
Key features and details
- Rabbit monoclonal [EPTPG1] to Nephrin (phospho Y1176 + Y1193)
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1]
See all Nephrin primary antibodies -
Description
Rabbit monoclonal [EPTPG1] to Nephrin (phospho Y1176 + Y1193) -
Host species
Rabbit -
Specificity
This antibody detects Nephrin when either tyrosines 1176 or 1193 are phosphorylated, but does not detect non-phosphorylated Nephrin. -
Tested applications
Suitable for: WB, Dot blotmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
-
Immunogen
Synthetic peptide within Mouse Nephrin (phospho Y1176 + Y1193). The exact sequence is proprietary.
-
Positive control
- Transfected HEK293T cells
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPTPG1 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)All lanes : Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299) at 1/1000 dilution
Lane 1 : HEK293T (human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin expression vector, whole cell lysate
Lane 2 : HEK293T (human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vector, whole cell lysate
Lane 3 : HEK293T (human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vector, whole cell lysate. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 138 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
-
![Dot Blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)](https://www.abcam.com/ps/products/80/ab80299/Images/ab80299-2-ab80299Dot1.jpg)
Dot Blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)Dot blot analysis of Nephrin (pY1176 + pY1193) phospho peptide (Lane 1), Nephrin (pY1176) phospho peptide (Lane 2), Nephrin (pY1193) phospho peptide (Lane 3) and Nephrin non-phospho peptide (Lane 4) labelling Nephrin (pY1176 + pY1193) with ab80299 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
-
![Western blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)](https://www.abcam.com/ps/products/80/ab80299/Images/Nephrin-Primary-antibodies-ab80299-1.jpg)
Western blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)All lanes : Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299) at 1/50000 dilution
Lane 1 : HEK293T (human embryonic kidney epithelial cell) transfected with wild type CD 16/7 Nephrin expression vector, whole cell lysate
Lane 2 : HEK293T (human embryonic kidney epithelial cell) transfected with wild type CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate
Lane 3 : HEK293T (human embryonic kidney epithelial cell) transfected with Y1176F mutant CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate
Lane 4 : HEK293T (human embryonic kidney epithelial cell) transfected with Y1193F mutant CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 138 kDa
Observed band size: 138,180 kDa why is the actual band size different from the predicted?
