Anti-Nephrin antibody (ab216692)
Key features and details
- Rabbit polyclonal to Nephrin
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat, Dog
- Isotype: IgG
Overview
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Product name
Anti-Nephrin antibody
See all Nephrin primary antibodies -
Description
Rabbit polyclonal to Nephrin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Dog
Predicted to work with: Human
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Immunogen
Synthetic peptide within Human Nephrin aa 450-550 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O60500 -
Positive control
- mouse kidney tissue; Rat RSC96 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nephrin antibody (ab216692)Anti-Nephrin antibody (ab216692) at 1/500 dilution + MDCK cell lysates
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody (ab216692)Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse kidney tissue labeling Nephrin using ab216692 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Flow Cytometry - Anti-Nephrin antibody (ab216692)Flow cytometric analysis of rat RSC96 cells labeling Nephrin using ab216692 at 1/100 dilution for 30 minutes (green), followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue) and isotype control (orange).
