Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5480] to PDGFR alpha - BSA and Azide free
  • Suitable for: IHC-P, WB, Flow Cyt, ELISA
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free
    See all PDGFR alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR5480] to PDGFR alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colonic carcinoma, human colon tissue. WB: SH SY5Y, C2C12, Human skeletal muscle, Human spleen, and Human fetal brain lysates. Flow Cyt: SH-SY5Y, L6 cells.

  • General notes

    Ab248689 is the carrier-free version of ab134123. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248689 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)

    This data was developed using ab134123, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling PDGFR alpha with ab134123 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells in human colonic carcinoma. The section was incubated with ab134123 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)
    Western blot - Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)
    All lanes : Anti-PDGFR alpha antibody [EPR5480] (ab134123) at 1/1000 dilution

    Lane 1 : Wild-type SH-SY5Y cell lysate
    Lane 2 : PDGFRA knockout SH-SY5Y cell lysate

    Lysates/proteins at 40 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 122 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab134123).

    Lanes 1 - 2: Merged signal (red and green). Green - ab134123 observed at 180 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab134123 was shown to react with PDGFR alpha in wild-type SH-SY5Y cells in Western blot with loss of signal observed in PDGFRA knockout sample. Wild-type SH-SY5Y and PDGFRA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab134123 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Flow Cytometry - Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)
    Flow Cytometry - Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)
    This data was developed using ab134123, the same antibody clone in a different buffer formulation.Flow cytometry analysis of L6(Rat skeletal muscle myoblast) cells labelling PDGFR alpha with ab134123 at 1/20 dilution (14.65 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).
  • Western blot - Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)
    Western blot - Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)
    All lanes : Anti-PDGFR alpha antibody [EPR5480] (ab134123) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Human spleen lysate
    Lane 3 : Human fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 122 kDa
    Observed band size: 190 kDa why is the actual band size different from the predicted?



    This data was developed using ab134123, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)
    Western blot - Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)
    All lanes : Anti-PDGFR alpha antibody [EPR5480] (ab134123) at 1/1000 dilution

    Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
    Lane 2 : C2C12 (mouse myoblast cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 122 kDa
    Observed band size: 190 kDa why is the actual band size different from the predicted?



    This data was developed using ab134123, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)

    This data was developed using ab134123, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PDGFR alpha with ab134123 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells in human colon (PMID: 23429582). The section was incubated with ab134123 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Flow Cytometry - Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)
    Flow Cytometry - Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)
    This data was developed using ab134123, the same antibody clone in a different buffer formulation.Flow cytometry analysis of SH-SY5Y(Human neuroblastoma epithelial cell) cells labelling PDGFR alpha with ab134123 at 1/20 dilution (14.65 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).
  • ELISA - Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)
    ELISA - Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)

    This data was developed using ab134123, the same antibody clone in a different buffer formulation.

    ELISA analysis of Human PDGFR alpha recombinant protein at 250 ng/mL with ab134123. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

  • Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)
    Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)

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