Recombinant human CSF-1-R protein (Active) (ab271478)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human CSF-1-R protein (Active)
See all CSF-1-R proteins and peptides -
Biological activity
Specific activity = 24 pmol/min/μg.
Assay buffer: 5 mM MOPS, 2.5 mM glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate with 0.25 mg/ml Poly (Glu:Tyr, 4:1) peptide substrate and [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
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Purity
>= 80 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
YKYKQKPKYQ VRWKIIESYE GNSYTFIDPT QLPYNEKWEF PRNNLQFGKT LGAGAFGKVV EATAFGLGKE DAVLKVAVKM LKSTAHADEK EALMSELKIM SHLGQHENIV NLLGACTHGG PVLVITEYCC YGDLLNFLRR KAEAMLGPSL SPGQDPEGGV DYKNIHLEKK YVRRDSGFSS QGVDTYVEMR PVSTSSNDSF SEQDLDKEDG RPLELRDLLH FSSQVAQGMA FLASKNCIHR DVAARNVLLT NGHVAKIGDF GLARDIMNDS NYIVKGNARL PVKWMAPESI FDCVYTVQSD VWSYGILLWE IFSLGLNPYP GILVNSKFYK LVKDGYQMAQ PAFAPKNIYS IMQACWALEP THRPTFQQIC SFLQEQAQED RRERDYTNLP SSSRSGGSGS SSSELEEESS SEHLTCCEQG DIAQPLLQPN NYQFC -
Predicted molecular weight
75 kDa including tags -
Amino acids
538 to 972 -
Tags
GST tag N-Terminus -
Additional sequence information
Genbank: BC047521
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Preparation and Storage
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Alternative names
- C FMS
- CD 115
- CD115
see all -
Function
Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34. -
Tissue specificity
Expressed in bone marrow and in differentiated blood mononuclear cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
Images
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Functional Studies - Recombinant Human CSF-1-R protein (Tagged) (ab271478)
Functional analysis of ab271478.
Specific activity = 24 pmol/min/μg.
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SDS-PAGE - Recombinant Human CSF-1-R protein (Tagged) (ab271478)SDS-PAGE analysis of ab271478.