Anti-Carbonic Anhydrase 8/CA8 antibody [008] - BSA and Azide free (ab276039)
![Anti-Carbonic Anhydrase 8/CA8 antibody [008] - BSA and Azide free (ab276039)](https://www.abcam.com/ps/products/276/ab276039/Images/ab276039-412815-AntiCarbonic-Anhydrase-8-CA8-antibody-008-wb-A549-cells.JPG "Anti-Carbonic Anhydrase 8/CA8 antibody [008] - BSA and Azide free (ab276039)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [008] to Carbonic Anhydrase 8/CA8 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Carbonic Anhydrase 8/CA8 antibody [008] - BSA and Azide free
See all Carbonic Anhydrase 8/CA8 primary antibodies -
Description
Rabbit monoclonal [008] to Carbonic Anhydrase 8/CA8 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human Carbonic Anhydrase 8/CA8 aa 1-290. NP_004047.3
Database link: P35219 -
Positive control
- IP: HeLa, A549 cells. WB: A549 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
008 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Carbonic Anhydrase 8/CA8 antibody [008] (ab276039)Anti-Carbonic Anhydrase 8/CA8 antibody [008] - BSA and Azide free (ab276039) at 1/500 dilution + A549 whole cell lysate at 30 µg
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/100000 dilution
Predicted band size: 33 kDa
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![Immunoprecipitation - Anti-Carbonic Anhydrase 8/CA8 antibody [008] (ab276039)](https://www.abcam.com/ps/products/276/ab276039/Images/ab276039-1-AntiCarbonic-Anhydrase-8-CA8-antibody-008-IP-A549-cells.jpg)
Immunoprecipitation - Anti-Carbonic Anhydrase 8/CA8 antibody [008] (ab276039)Lane A: 0.5 mg HeLa Whole Cell Lysate
Lane B: 0.5 mg A549 Whole Cell Lysate
Carbonic Anhydrase 8/CA8 wasmunoprecipitated from 0.5 mg whole cell lysate using 0.5 µL ab276039 and 15 µl of 50 % Protein G agarose.
Primary antibody: ab276039, at 1/150 dilution
Secondary antibody used Dylight 800-labeled antibody to rabbit IgG (H+L), at 1/5000 dilution.Performed under reducing conditions.
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![Anti-Carbonic Anhydrase 8/CA8 antibody [008] - BSA and Azide free (ab276039)](https://www.abcam.com/ps/products/276/ab276039/Images/ab276039-3-benefits-of-recombinant-antibodies.png)
Anti-Carbonic Anhydrase 8/CA8 antibody [008] - BSA and Azide free (ab276039)