Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)
![Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)](https://www.abcam.com/ps/products/240/ab240925/Images/ab240925-384886-anti-bcl6-antibody-ep529y-bsa-and-azide-free-western-blot-hela-ramos-sh-sy5y.jpg "Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP529Y] to Bcl6 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
-
Product name
Anti-Bcl6 antibody [EP529Y] - BSA and Azide free
See all Bcl6 primary antibodies -
Description
Rabbit monoclonal [EP529Y] to Bcl6 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Daudi and Ramos, HeLa and SH-SY5Y cell lysates.
-
General notes
ab240925 is the carrier-free version of ab33901 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab240925 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP529Y -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)All lanes : Anti-Bcl6 antibody [EP529Y] (ab33901) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : BCL6 knockout HeLa cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 78 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab33901).
Lanes 1-4: Merged signal (red and green). Green ab33901 observed at 78 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab33901 Anti-Bcl6 antibody [EP529Y] was shown to react with BCL6 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265410 (knockout cell lysate ab257178) was used. Wild-type and Bcl6 knockout samples were subjected to SDS-PAGE. ab33901 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)](https://www.abcam.com/ps/products/240/ab240925/Images/ab240925-330681-anti-bcl6-antibody-ep529y-western-blot.jpg)
Western blot - Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)All lanes : Anti-Bcl6 antibody [EP529Y] (ab33901) at 1/1000 dilution
Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method
Lane 2 : Ramos (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 78 kDa
Exposure time: 10 secondsBlocking and diluting buffer: 5% NFDM/TBST.
This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab33901).
-
![Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)](https://www.abcam.com/ps/products/240/ab240925/Images/ab240925-3-benefits-of-recombinant-antibodies.png)
Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)
