Antibodies, Proteins, Kits and Reagents for Life Science

465 705 ₸ Product size

1 ml 465 705 ₸ 500 µl 385 296 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Bcl6 antibody [SP155] - C-terminal (ab183308)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP155] to Bcl6 - C-terminal
Suitable for: WB, Flow Cyt, IHC-P
Reacts with: Human

Product name

Anti-Bcl6 antibody [SP155] - C-terminal
See all Bcl6 primary antibodies
Description

Rabbit monoclonal [SP155] to Bcl6 - C-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human tonsil, B-cell lymphoma, HK lymphoma, and follicular lymphoma tissue. WB: Ramos cell lysate.
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP155
Isotype

IgG
Research areas

Western blot - Anti-Bcl6 antibody [SP155] - C-terminal (ab183308)

All lanes : Anti-Bcl6 antibody [SP155] - C-terminal (ab183308) at 0.135 µg/ml

Lane 1 : Ramos(Human Burkitt's lymphoma B lymphocyte)
Lane 2 : Raji( Human Burkitt's lymphoma B lymphocyte)
Lane 3 : Daudi( Human Burkitt's lymphoma lymphoblast)
Lane 4 : HEK-293( Human embryonic kidney epithelial cell)

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml ("Goat Anti-Rabbit IgG, (H+L),
Peroxidase conjugated ")

Predicted band size: 79 kDa
Observed band size: 78 kDa
why is the actual band size different from the predicted?

Blocking/Diluting Buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - C-terminal (ab183308)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Bcl6 with ab183308 at 1/100 dilution (1.35 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - C-terminal (ab183308)

Immunohistochemical analysis of paraffin-embedded Human B-cell lymphoma tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - C-terminal (ab183308)

Immunohistochemical analysis of paraffin-embedded Human follicular lymphoma tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - C-terminal (ab183308)

Immunohistochemical analysis of paraffin-embedded Human HK lymphoma tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - C-terminal (ab183308)

Immunohistochemical analysis of paraffin-embedded Human B-cell lymphoma tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - C-terminal (ab183308)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - C-terminal (ab183308)

Immunohistochemical analysis of paraffin-embedded Human B-cell lymphoma tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - C-terminal (ab183308)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.
Flow Cytometry - Anti-Bcl6 antibody [SP155] - C-terminal (ab183308)

Flow cytometry analysis of Human Ramos cells labeling Bcl6 (green), using ab183308, and negative control of Rabbit IgG (blue)
Anti-Bcl6 antibody [SP155] - C-terminal (ab183308)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com